GLN缓解中华鳖免疫应激反应机制的初步研究

PROTECTION AGAINST IMMUNOLOGICAL STRESS BY INJECTING GLN INTO TRIONYX SINENSIS

本文探讨GLN缓解中华鳖(Trionyx sinensis)免疫应激反应的作用机制,为通过营养调控手段增强机体免疫保护作用,促进水生动物健康养殖提供依据。试验选择体重接近的健康中华鳖60只,随机分为3组,分别腹腔注射0.7%NaC l、500μg LPS/Kg BW、500μg LPS+100mg GLN/Kg BW。检测不同时段血浆COR、TNF-α、IL-1β、IL-6、IgM、IgG、IgA、LSZ、GSH、GSH-PX、MDA以及肝脏PRO、GSH、GSH-PX、MDA含量的变化。结果表明:注射LPS引起中华鳖产生了免疫应激反应,血浆中COR、TNF-α、IL-1β、IL-6、IgM、GSH-PX、MDA和肝脏组织PRO、GSH-PX、MDA含量在相应时段显著升高(p<0.05),而血浆和肝脏GSH含量显著降低(p<0.05);补充GLN不仅缓解了COR、TNF-α、IL-1β和IL-6等的上升趋势,而且使非特异性免疫抑制剂(MDA)水平降低。LPS作为免疫原可引起中华鳖产生免疫应激反应,补充GLN可以缓解免疫应激,对中华鳖具有免疫保护作用。

Trionyx sinensis are highly nutritious and tonic. However,They are bred such as artificial regulation of temperature and densely intensive aquiculture has changed the natural habits and characteristics of Trionyx sinensis. As a consequence, Trionyx sinensis are less able to resist disease, and more liable to specific and non-specific antigen, which places Trionyx^sinensis frequently under immunological stress. Being a conditioned essential amino acid, Glutamine（GLN） is not only nourishing, but also effective in pharmacological function, such as maintaining intestinal function, and enhancing organism＇s immunity against stress, oxidation and infection. By studying the protection against immunological stress of Trionyx sinensis GLN provides, this paper aims to form basis for the improvement of immunity against diseases by means of nutrition regulation and healthy aquiculture of aquatic animals. This experiment made a study of sixty Trionyx sinensis with similar weight （339.46 ± 25.54g）, which were randomly divided into three groups：twelve in Group A, twenty-four in Group B and Group C respectively. 0. 7% Nacl,500μg Lipopolysaccharide （LPS）/kg BW and 500μg LPS ＋ 100mg GLN/ kg BW were injected into Group A,Group B and Group C respectively. LPS and LPS plus GLN were combined into 0.7% Nacl for injection separately, with the dose of 0.5mL in each subject. Radio-immunoassay （FJ-2008 γ immunocount instrument） were used to detect the content change of Cortisol （ COR）, Tumor necrosis factor-α（ TNF-α）, Interleukin-1β （ IL- 1β） and Interleukin-6 （ IL-6 ） in, the plasma at certain times （5h, 1 d, 4d and 7d after injection） ; and pectrophotometer （Unico2000） were used to measure the content change of Immunoglobulin M （IgM）, Immunoglobulin G （IgG）, Immuno- globulin A （ IgA ）, Lysozyme （ LSZ ）, Glutathione （ GSH ）, Glutathione peroxidase （ GSH-PX ）, Malondialdehyde （ MDA ） in blood plasma, and that of Protein （PRO） , GSH, GSH-PX and M DA in liver at different times. The results are following：Tri- onyx sinensis are capable of immunological stress by injecting LPS,with rapid increase in the content of COR,TNF-α, IL- 1β, IL-6, IgM, GSH-PX, MDA in blood plasma and PRO, GSH-PX, MDA in liver（p 〈 0.05 ）, but sharp decline in the content of GSH in blood plasma and liver at corresponding times（p 〈0. 05）. The supplement of GLN not only slows down the rise of COR, TNF-α, IL-1β and IL-1 which arising from the injection of LPS,but also decreases the content of non-specific immunological inhibitor MDA in blood plasma,and the content of MDA in liver declined remarkably（p1d 〈 0.05;p4d 〈 0.01）. In conclusion, the change in neuroendocrine, immunity and anti-oxidation owing to the injection of LPS indicates that the supplement of GLN can relieve the immunological stress,showing that the injection of GLN can provide protection of immunological stress of Trionyx sinensis.