胰腺癌组织DNA倍体与其生物学行为的关系

Relationship between DNA ploidy analysis and biological behavior in pancreatic tumor

目的探讨胰腺癌组织中的DNA倍体与其生物学行为关系。方法应用流式细胞术测定30例胰腺癌患者肿瘤组织DNA含量,分析DNA倍体与胰腺癌患者的临床分期、病理分级以及肿瘤转移的关系。结果胰腺癌组织中DNA倍体异倍体检出率及增殖指数与患者的年龄、性别以及肿瘤大小无统计学意义(P>0.05),异倍体检出率在TNM分期和淋巴结转移中差异存在统计学意义(P<0.05)。结论胰腺癌组织中DNA倍体异质性及增殖指数能有效地反映肿瘤的增殖情况和恶性程度,并且与临床生物学行为关系密切,DNA倍体的检测可以作为指导治疗和改善预后的重要指标。

Objective To evaluate the clinical value of DNA ploidy in pancreatic cancer, Methods The DNA ploidy of tumor tissue was measured in 30 patients with pancreatic cancer using flow cytometry, The relationship were analyzed among DNA ploidy and clinical stage, pathological grade, metastasis rate of patients with pancreatic cancer. Results Flow cytometry DNA Ploidy and PI were no signifcantly correlated with age, gender and tumor size, and were significantly correlated with TNM stage and LN metastasis. Conclusion The analysis of DNA Ploidy and PI in pancreatic cancer patients can availably reflect the instance of the cell multiplication and the degree of cancer, and closely relate to their clinical biological behaviors. Detection of DNA ploidy can be used as an important index for instruction of the treatmend and improving the prognosis.