三氧化二砷对肝癌细胞株SMMC-7721[Ca^(2+)]i的影响

Effect of As_2O_3 on intracellular Ca^(2+) in SMMC-7721 cell line

目的观察不同浓度三氧化二砷(arsenic trioxide,As2O3)对人肝癌细胞株SMMC-7721增殖及细胞内Ca2+的影响,探讨其在肝癌化疗中的可能机制。方法MTT法测定不同浓度As2O3对SMMC-7721细胞的抑制作用;Fura2-AM标记细胞内Ca2+,荧光分析仪测定不同浓度As2O3对SMMC-7721细胞内Ca2+浓度的影响。结果As2O3在0.5～2.0μg/ml的浓度范围内对肝癌SMMC-7721细胞的生长具有抑制趋势,但差异无统计学意义(P>0.05);As2O3在0.5,1.0μg/ml浓度时,对SMMC-7721细胞内Ca2+浓度的影响,与对照组相比差异无统计学意义(P>0.05);当As2O3浓度为2.0μg/ml,作用时间为48h,与对照组相比差异有统计学意义(P<0.01),而作用时间至72h,细胞内Ca2+又恢复至正常水平(P>0.05)。结论As2O3对SMMC-7721细胞内Ca2+的影响不仅与浓度有关而且与作用时间有关。

Objective To observe the effect of the different concentrations of As203 on proliferation and intracellular Ca2＋ in SMMC-7721 cell line, and discuss its mechanism. Methods Cell proliferation was evaluated with MTT： [Ca2＋]i was labeled with a fluorescence probe Fura2-AM and measurement of fluorescence was monitored with a Thermo Labsystems Fluoroskan Ascent, USA. Results There was an inhibition trend on SMMC-7721 cell proliferation when the concentration of As203 ranged from 0.5 to 2.0 μg/ml, but the difference was not significant （P〉0.05）. There was no significant difference of [Ca2＋]i, when the cells were treated with As203 0.5 and 1.0 μg/ml, respectively; When the cells were treated with As203 2.0 μg/ml for 48 h, the concentration of intracellular Ca2＋ was statistically different from that in the control group cells （P〈0.01）. After 72 h of As203 treatment, however, the levels of intracellular Ca2＋ were not statistically different from that in control group cells （P〉0.05）. Conclusion The effect of As2O3 on SMMC-7721 displays a dose- and time dependent manner.