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利用体细胞核移植技术生产黄牛和水牛同种、异种转基因克隆囊胚 被引量：3

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摘要 以含neo和GFP基因双标记的水牛、黄牛胎儿成纤维细胞进行牛转基因体细胞核移植,结果发现:阿菲迪霉素可有效将胎儿成纤维细胞同步于G0/G1期;以GFP阳性细胞进行核移植,其卵裂率、囊胚率与对照组间无显著差异(P>0.05),所构建的重组胚,2-细胞阶段观察不到GFP的表达,4-细胞以后GFP的表达逐渐增强,在囊胚的内细胞团和滋养层细胞均可检测到GFP的表达,将黄牛同种转基因克隆囊胚进行移植,获得1例妊娠;黄牛卵母细胞一水牛供核细胞构建异种重组胚囊胚率显著高于黄牛供核细胞一水牛卵母细胞构建的异种重组胚(P<0.05),并且异种核移植重组胚中可检测到GFP表达,GFP可作为异种核移植胚胎来源的一种新标记.
作者 韦精卫 孟凡丽 韦英明 杨素芳 陆凤花 石德顺
机构地区 广西大学动物繁殖研究所
出处 《自然科学进展》 北大核心 2008年第8期857-862,共6页
基金 国家"八六三"高科技研究发展计划(2007AA100505) 广西青年科学基金(桂科青0640008) 广西大学博士启动基金(DD150034)资助
关键词 水牛 黄牛 绿色荧光蛋白(GFP) 转基因体细胞 种间核移植
分类号 S814.8 [农业科学—畜牧学] Q785 [生物学—分子生物学]
  • 相关文献

参考文献26

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二级参考文献16

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共引文献25

同被引文献54

  • 1鞠丽梅,三好一郎,笠井憲雪,蔡有余.显微注射法制备转基因小鼠的技术研究[J].中国实验动物学报,1999,7(1):11-15. 被引量：9
  • 2陆凤花,石德顺,谢英,韦英明,潘红平,韦精卫,卞桂华,成俊萍,王晓丽.不同来源的供体细胞对水牛体细胞核移植效果的影响[J].广西农业生物科学,2005,24(2):89-93. 被引量：6
  • 3张运海,潘登科,孙秀柱,孙国杰,王晓波,刘晓辉,李燕,戴蕴平,李宁.利用体细胞核移植技术生产表达绿色荧光蛋白的猪转基因克隆胚胎[J].中国科学（C辑）,2005,35(5):439-445. 被引量：21
  • 4王海,敖红,潘求真,李荣旗,赵孟彬,连正兴,李宁,吴常信.绵羊胎儿成纤维细胞不同处理对核移植重构胚发育的影响[J].中国科学（C辑）,2006,36(5):398-404. 被引量：2
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引证文献3

二级引证文献4

自然科学进展
2008年 第8期

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