葡聚糖T20作为白芨多糖含量测定标准品可行性研究

The study on feasibility of determinating the polysaccharide isolated from bletilla striata using dextron T20 as standard substance

目的:采用星点设计-效应面法(Central composite design-response surface methodology,CCD-RSM),考察蒽酮-硫酸法测量白芨多糖含量的可行性和适用条件。方法:以葡聚糖T20为标准品,以多糖溶液与蒽酮试剂比例、水解显色时间、室温冷却时间为自变量,621.0nm处的吸光度为因变量,对自变量各水平进行多元线性回归和二项式拟合,考察因变量和自变量间的相互关系,采用效应面法优化蒽酮-硫酸法测量白芨多糖含量的适用条件。结果:当多糖溶液与蒽酮试剂比例为1:2,水解显色时间8.00min,室温冷却放置15.00min时,测定葡聚糖T20和白芨多糖含量具有良好的相关性。结论:星点设计-效应面法可以用于评估以葡聚糖T20为标准品、蒽酮-硫酸法测量白芨多糖含量的可行性,并优化测量含量的适用条件。

Objective：To optimize the condition of anthrone-sulfuric acid method to determine the polysaccharide isolated from Bletilla striata by central composite design/response surface methodology. Methods：Dextron T20 was a standard substance, independent variables were the ratio of the polysaccharide and anthrone-sulfuric acid,the time of color hydrolysis and the cooling time at room temperature,dependent variable was the UV absorption, nonlinear mathematic models were used to estimate the relationship between independent and dependent variables. Response surface methodology was used to optimize he condition of determination. Results：The optimum conditions of determination.the volume ratio of the polysaccharide and anthrone-sulfuric acid is l：2;the time of color hydrolysis is 8.00 min;the cooling time at room temperature is 15.00min. On this condition, there was favorable correlation between the absorption of Dextron T20 and polysaccharide of Bletilla striata. Conclusions： Central composite design/response surface methodology can be used to evaluate the feasibility and applicable conditions of the anthrone-sulfuric acid method to determine the polysaccharide isolated from Bletilla striata using Dextron T20 as a standard substance.