树突状细胞对海藻酸钙纳米胶囊的吞噬作用与功能诱导

Endocytosis of Ca Alginate Nanocapsules by Dendritic Cells and Function Induction

采用量子点标记方法,证实了人外周血来源树突状细胞对海藻酸钙纳米胶囊的吞噬作用,并对其体外成熟诱导和接受偶联有牛血清白蛋白的纳米胶囊刺激之后的自体T淋巴细胞免疫作用进行了探讨。结果表明,树突状细胞在吞噬海藻酸钙纳米胶囊后被诱导成熟,而且偶联有牛血清白蛋白的纳米胶囊可诱导自体T淋巴细胞增殖,显示该海藻酸钙纳米胶囊可能成为具有载体功能的癌症细胞免疫治疗佐剂。

The endocytosis/phagocytosis of calcium alginate nanocapsules by peripheral blood derived dendritic cells was confirmed with the use of Quantum dots labeling. Results further demonstrated these nanoparticles could cause the upregulation of HLA-DR expression to induce the maturation of dendritic cells in vitro. Dendritic cells stimulated by nanocapsules covalently loaded with BSA were shown to stimulate the proliferation of self T cells with blank capsules and free BSA as controls, suggesting their potential applications in cancer cell therapy as a new antigen delivery vehicle with a strong adjuvant effect.