猪圆环病毒2型-细小病毒-伪狂犬重组病毒免疫小鼠试验研究

Study on the Immunogenicity of Recombinant Virus of Pseudorabies-porcine Circovius Type 2-porcine Parvovirus

为了研究表达猪2型圆环病毒ORF2基因和猪细小病毒VP2基因的重组伪狂犬病毒疫苗株SA215(D)的疫苗价值,对该病毒株安全性、免疫原性、保护力及对PRVFa株定值进行了研究。研究结果表明重组病毒SA215(D)毒力显著低于PRVFa强毒株,可以抵御伪狂犬病强毒Fa株的攻击,并能抵御其在小鼠体内的定植;抗体检测显示SA215(D)免疫小鼠后第2周抗PRV、PCV2和PPV的抗体水平均很低,加强免疫后第4周抗体水平大幅上升,且显著高于阴性对照组,而与阳性对照组无明显差异,表明SA215(D)可诱导小鼠产生体液免疫反应。淋巴细胞增殖试验显示SA215(D)可诱导小鼠产生较强的淋巴细胞增殖反应,与亲本株SA215组相比,差异显著(0.01<P<0.05),与PBS对照组相比较差异极显著(P<0.01)。为开发具有应用前景的三价基因工程疫苗奠定基础。

The viral safety, immunogenicity, and the protection against PRV Fa strain of the recombinant pseudorabies virus SA215 （ D ） expressing ORF2 gene of porcine circovius type 2 and VP2 gene of porcine parvovirus were conducted to study its application value as the vacine strain. The result indicated that SA215 （D） had lower virulence than velogenic strain velogenic strain which could resist the attack of PRV Fa strain. The ELISA test showed the levels of antibody against PRV, PPV and PCV2 of mice vaccined with SA215 （D） were low after 2 weeks, and the levels increased significantly at the fourth week after strengthening immunization, which indicated SA215 （D） could induced humoral immune response in mice. The test of lymphocytes proliferation showed that SA215 （D） could induced cell immune response in mice, and it was significant difference comparied with parent plant of SA215 and control group of PBS. The foundation for the development of trivalence genetically engineering vaccine was established was established.