摘要
OsRacD是光敏核不育水稻农垦58S光周期育性转换信号通路中的重要分子开关,通过酵母双杂交筛选,从水稻幼穗中分离到一种与OsRacD互作的新的肌球蛋白重链编码基因的cDNA片段,命名为OsMY1。在酵母双杂交体系鉴定的基础上,构建了OsMY1和OsRacD的原核表达载体,表达和纯化了融合蛋白GST-OsMY1和His6-OsRacD,通过pulldown实验进一步确认了上述互作关系。提示OsMY1和OsRacD可能在功能上相关,为深入研究二者在水稻生理功能上的联系提供了重要线索和依据。
OsRacD is a critical molecular switch sensitive genic male sterile rice. Taking OsRacD as involved in photoperiod fertility conversion of photoperiod bait, a new rice myosin heavy chain partial eDNA was isolated from the rice panicle by yeast two-hybrid, and designated OsMY1. The interaction between OsMY1 and OsRacD had been testified by yeast two-hybrid and pull down assay, it showed that OsMY1 could bind with OsRacD,suggested that OsMY1 maybe the target of OsRacD. The important evidence for further study on the functional relationship between OsMY1 and OsRacD in vivo are also provided.
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2008年第7期63-66,共4页
China Biotechnology
基金
河南省科技攻关项目(NO0624440060)
