真菌性角膜溃疡两种实验室诊断方法的比较

Comparison between two kinds of laboratory diagnosis methods of fungal keratohelcosis

目的:寻找快速,简便、准确诊断真菌性角膜溃疡的实验室诊断方法。方法:采用角膜刮片取材,涂片后进行KOH湿片直接镜检法及丫啶橙荧光染色法同时检测140例临床疑似真菌性角膜溃疡患者的标本,对结果进行比较。结果:KOH湿片直接镜检法检测阳性率为71%,丫啶橙荧光染色法阳性率为86%,结果比较有统计学意义(P<0.05)。结论:丫啶橙荧光染色法简便,快速、准确,阳性率高,优于KOH湿片直接镜检法。随着目前国产荧光显微镜的日益普及,我们认为该方法为快速检出真菌的好方法,值得推广应用。

AIM： To found a quick, simple, and exact laboratory diagnosis method of fusarium keratohelcosis. METHODS： Samples from 140 cases with suspected fungal keratohelcosis were adopted corneal scraping, and then detected by KOH microscopic examination and acridine orange staining fluorescence. The results were compared. RESULTS： The positive rate of KOH microscopic examination and acridine orange staining fluorescence were 71% and 86%, respectively, and had statistical significance （ P〈 0.05）. CONCLUSION： Characterized by quick, simple, exact, and high positive rate, acridine orange staining fluorescence is better than KOH microscopic examination on diagnosis of fungal keratohelcosis. With the popularization of homemade fluorescence microscope, we believe that acridine orange staining fluorescence would be good way for fungal detection and is worth promoting.