原发系膜增殖性肾小球肾炎患者中性粒细胞和内皮细胞活化状态研究

Expression of CD11a, CD11b and CD62L genes in Mesangial Proliferative Glomerulonephritis

目的系膜增殖性肾小球肾炎主要表现为肾病综合征、血尿及蛋白尿三种类型。探讨原发系膜增殖性肾小球肾炎（MsPGN）患者外周血及肾组织CD11a、CD11b、CD62L的表达，探讨原发系膜增殖性肾小球肾炎（MsPGN）患者外周血及肾组织CD11a、CD11b、CD62L的表达。方法采用流式细胞技术检测35例MsPGN患者外周血及免疫组织化学染色标记肾组织CD11a、CD11b、CD62L的表达情况。结果MsPGN患者外周血CD11a、CD11b明显低于正常，而CD62L显著升高。CD11b降低程度与CD11a相一致，且CD11b降低程度与CD62L呈负相关关系（r=-0．263，P〈0．05）。肾组织CD11a、CD11b、CD62L呈阳性表达。结论MsPGN患者外周血及肾组织的CD11a、CD11b、CD62L存在异常，提示CD11a、CD11b、CD62L在MsPGN的发病中可能具有一定的作用。

Objective The person＇s Mesangial Proliferative Glomerulonephritis （MsPGN） were divided into 3 types based on clinical manifestations： nephroticsyndrome, hematuria and proteinuria. To investigate the expression of CD11a, CD11b, CD62L and its signifi-cance in the kidneys patients with MsPGN. Methods CD11a, CD11b and CD62L expression in blood of MsPGN patients （ n = 35） were investigated by flow cytometry method, and the changes of these proteins in kidney were surveyed. Results In MsPGN, CD11a and CD11b expression in blood were significantly lower and CD62L expression in blood were markedly higher than that in normal humans. And the renal glomeruli of MsPGN also expressed CD11a, CD11b and CD62L. Conclusion The expression of CD11a, CD11b and CD62L are abnormal in MsPGN, and apoptosis may play certain role in the pathogenesis of MsPGN.