凋亡抑制蛋白Survivin在人正常胎盘滋养细胞中的表达及其与Bcl-2、Bax的关系

Expression of survivin protein and its relation with the expression of Bcl-2 and Bax in the trophoblasts of human normal placenta

目的探讨凋亡抑制蛋白Survivin在人正常各期胎盘滋养细胞中的表达，及其与Bcl-2、Bax表达的关系。方法将30例孕妇分为早孕组（10例），中孕组（10例）和足月组（10例），取正常妊娠8-9周、18-23周、37-40周胎盘组织固定，石蜡包埋，采用免疫组织化学方法检测各期胎盘滋养细胞中Survivin、Bcl-2及Bax的表达情况。结果Survivin在3组胎盘滋养层的表达随孕期的增长逐渐减弱（Pu值分别为：11.74±0.8，9.95±0.43，8.83±0.67，P〈0.01）；相反，Bcl-2和Bax均随孕期的增长逐渐增加（Bcl-2的Pu值分别为：4.33±0.60，5.00±0.75，6.87±0.45。P〈0.01；Bax的PU值分别为：9．82±1．12，16．00±1．05，27．48±2．10，P〈0．01）；Bcl-2、Bax与Survivin均呈无相关（P〉0．05）。结论Survivin可能通过抑制滋养细胞凋亡的途径参与了正常妊娠胎盘的发育。Survivin的表达与Bcl-2、Bax表达无相关，提示抑制细胞凋亡的过程中，它们是通过不同的途径发挥各自的生物学作用。

Objective To study the expression of Survivin protein and its relation with the expression of Bcl-2, Bax in the tropho-blasts of human normal placenta. Methods The normal placental tissues （8-9week , 18-23 week and 37-40week） were fixed, embedded, sectioned, and Survivin, Bcl-2, and Bax in the trophoblasts were detected with immunnohistochemistry. Result As the gestational age advanced , the staining intensity of Survivin in the trophoblasts was significantly decreased from the first trimester group to the second trimester group to the term group（ PU value： 11.74±0.8,9.95±0.43,8.83±0.67,respectively, P 〈0.01）, while the staining intensity of Bcl-2 and Bax in trophoblast was significantly increased （PU value of Bcl-2 ： 4.33±0.60, 5.00±0.75,6.87±0.45 ,respectively, P 〈0.01 and PU value of Bax： 9.82±1.12,16.00± 1.05,27.48 ±2.10, respectively, P 〈0.01）. Expression of Survivin in trophoblasts has no rela- tionship with the expression of Bcl-2 and Bax （ P 〉0.05 ）. Conclusion Survivin may take part in the development of human normal pla- centa through the way of suppressing the apoptosis in trophoblasts. Expression of Survivin in trophoblasts has no relationship with the expression of Bcl-2 and Bax, which indicate that they regulate apoptosis of trophoblasts via different biological pathways.