摘要
以花生品种花育20成熟胚萌发5~7d的幼叶为外植体,利用农杆菌介导法进行几丁质酶基因(CH5B)的遗传转化,并对转化条件进行了研究。结果表明,外植体经预培养2d,于OD600=0.6~0.8的农杆菌(GV3101)菌液中浸泡15min,再共培养3d,最适宜遗传转化。对抗性植株进行PCR检测,扩增得到了目的条带,表明几丁质酶基因成功转入了花生。
CH5B gene was transferred into peanut cultivar Huayu No. 20 mediated by Agrobacterium tumefaciens , and transformation conditions were studied in this paper. The result showed that pre - culture for 2 days, Agrobacterium suspension (OD600 =0.6-0.8) for 15 min and co-culture for 3 days was suitable to obtain resistant shoots. The target band of resistant plants was amplified by PCR analysis. These results confirmed that CH5B gene was transferred into peanut successfully.
出处
《青岛农业大学学报(自然科学版)》
2008年第2期84-87,共4页
Journal of Qingdao Agricultural University(Natural Science)
基金
国家公益性行业科研专项项目(nvhyzx07-014)
国家自然基金项目(30270837)