一种聚丙烯酰胺凝胶的快速染色方法——热考染法

A rapid staining method of polyacrylamide gel electrophoresis——heated CBB staining

在蛋白的染色方法中,目前以考马斯亮蓝染色最为常用。以樟子松的针叶为材料,以加热的考马斯亮蓝R-350染液和未经加热的考马斯亮蓝R-350染液以及未经加热的考马斯亮蓝R-250染液进行染色法方法的比较,从而证明热考染法具有蛋白点清晰、背景及对比度良好、安全、操作便捷等较优的染色特点。

In current protein staining method, Coomassie Brilliant Blue （CBB） staining is the most common one. The pine needles of the Pinus sylvestris were used as experiment materials. Compared with Coomassie Brilliant Blue R -250 and unheated Coomassie Brilliant Blue R -350. It is proved that the heated Coomassie Brilliant Blue R - 350 has the optimum staining characteristics in protein spots＇ clarity, background, contrast, safety, convenient op- eration and so on.