基于发光共轭聚合物的核酸生物传感器
Flurometric assays for DNA and protein with conjugated polyelectrolytes
摘要
本文介绍了以发光共轭聚合物为基础,以荧光为检测手段,针对核酸进行特异性识别的一类检测信号放大的生物传感器。主要介绍了DNA和RNA的几种检测方法,探讨了影响荧光传感信号的因素。
出处
《生命科学》
CSCD
2008年第3期383-389,共7页
Chinese Bulletin of Life Sciences
基金
中国科学院"百人计划"项目
参考文献69
-
1Wang J. From DNA biosensors to gene chips.Nucleic Acids Res, 2000, 28:3011-6
-
2Umek RM, Lin SW, Viclmetter J, et al. Electronic detection of nucleic acids: A versatile platform for molecular diagnostics. J Mol Diag, 2001, 3:74-84
-
3Schork NJ, Fallin D, Lanchbury JS. Single nucleotide polymorphisms and the future of genetic epidemiology. Clin Genet, 2000, 58:250-64
-
4Balakin KV, Korshun VA, Mikhalcv II, et al. Conjugates of oligonucleotides with polyaromatic fluorophores as promising DNA probes1. Biosens Bioelectron,1998,13:771-8
-
5McQuade DT, Pullen AE, Swager TM. Single nucleotide polymorphisms and the future of genetic epidemiology. Chem Rev, 2000, 10:2537-74
-
6Dybertret BM, Calame M, Libchaber A. Single-mismatch detection using gold-quenched fluorescent oligonucleotides. Nat Biotechnol, 2001,19:365-70
-
7CarduUo RA, Agrawal S, Flores C, et al. Detection of nucleic acid hybridization by nonradiative fluorescence resonance energy transfer. Proc Natl Acad Sci USA, 1988, 85: 8790-4
-
8Xu XH, Bard AJ. Immobilization and hybridization of DNA on an aluminum(Ⅲ) alkanebisphosphonate thin film with electrogenerated chemiluminescent detection. J Am Chem Soc, 1995, 117:2627-31
-
9Peterlinz K, Georgiadis R, Heine T, et al. Observation of hybridization and dehybridization of thiol-tethered DNA using two-color surface plasmon resonance spectroscopy. J Am Chem Soc, 1997, 119:3401-2
-
10Chen LH, McBranch DW, Wang HL, et al. Highly sensitive biological and chemical sensors based on reversible fluorescence quenching in a conjugated polymer. Proc Natl Acad Sci USA, 1999, 96:12287-92
-
1朱滨,王国荃.核酸生物传感器及其研究进展[J].生物化学与生物物理进展,1997,24(6):510-513. 被引量:1
-
2MED的eyescreen赢得了视频眼镜设计[J].家电大视野,2008(5):94-94.
-
3吴永强.DNA生物传感器的研究进展[J].四川轻化工学院学报,2003,16(4):31-36. 被引量:1
-
4林虹君,张爱红,吉会海,宫金萍.纳米材料作为载体在检测信号放大中的应用进展[J].生物技术通讯,2015,26(1):154-158.
-
5蒋庆全.新型纸状显示器发展浅析[J].电子科学技术评论,2004(5):25-31.
-
6Tsukasa Suzuki,Ken Inoki.Spatial regulation of the mTORC1 system in amino acids sensing pathway[J].Acta Biochimica et Biophysica Sinica,2011,43(9):671-679. 被引量:9
-
7崔刚,陈文楷.基于CPLD的温度控制系统[J].现代电子技术,2005,28(21):68-69. 被引量:1
-
8TANG ZhiYuan CHENG YongQiang DU Qing ZHANG HongXia LI ZhengPing.Integration of rolling circle amplification and cationic conjugated polymer for the homogeneous detection of single nucleotide polymorphisms[J].Chinese Science Bulletin,2011,56(31):3247-3252. 被引量:3
-
9XING BaoLing,SONG JinZhao,GE SuMei,TANG ZhengHua,LIU MengLu,YANG Qiong,Lü FengTing,LIU LiBing,WANG Shu.Multiplex detection of KRAS and BRAF mutations using cationic conjugated polymers[J].Chinese Science Bulletin,2013,58(8):873-878. 被引量:1
-
10傅妮娜,赵保敏,汪联辉,黄维.基于水溶性共轭聚合物的蛋白质荧光传感器[J].南京邮电大学学报(自然科学版),2008,28(1):70-76. 被引量:2