胆固醇脂质体对培养兔胆道口括约肌细胞骨架的作用

Effects of cholesterol liposome on cytoskeleton of rabbit sphincter of Oddi cells in culture

研究胆固醇对胆道口括约肌（SO）细胞动力改变的结构基础，进一步解释胆固醇结石形成的原因．方法：分离幼兔SO采用复合酶消化法行原代培养α－平滑肌肌动蛋白（α－SMActin）染色鉴定，人工制作胆固醇脂质体（CL），按不同浓度作用细胞后行显微镜、原位包埋电镜观察及电泳测定．结果：电镜示CL组部分细胞器肿胀、扩张，微丝有明显分开、减少的趋势，电泳示随胆固醇浓度增高细胞蛋白（包括Actin）含量减少．结论：胆固醇首先作用于SO细胞膜，改变其流动性及通透性，继而可以直接作用于细胞器及微丝结构，通过改变细胞骨架的构型及含量而引起SO动力改变．

To explain the causes of cholesterol stone by the study of structural changes of sphincter of oddi (SO) cells under cholesterol liposome (CL) effects. Methods: We isolated rabbit SO to perform primary cell culture and observed the SO cell changes which were affected by different concentration CL through the technique of electron microscope and electrophoresis. Results: Electron microscopy results showed mitochodria swelled and endoplasmic reticulum dilated as well as microfilament disjoined and diminished in some cells. Electrophoresis indicated SO cell protein (include Actin) content decreased with the increasement of cholesterol concentration. There was a significant difference between CL groups and control group (P＜0. 01 ). Conclusion:CL can change SO cell membrane fluidity and permeability, then affect SO cell organelle's structure and especially change the quantity and configuration of microfilaments, which may depress SO motility.