阿魏酸哌嗪对糖尿病大鼠肾脏的保护作用

The Effect and its Mechanism of Piperazine Ferulate on STZ-induced Diabetic Nephropathy in Rats

目的:观察阿魏酸哌嗪对糖尿病大鼠肾脏的保护作用。方法:将雄性SD大鼠32只随机分为4组:正常组、模型组、阿魏酸哌嗪治疗组、厄贝沙坦治疗组,采用STZ诱导的糖尿病大鼠模型,阿魏酸哌嗪组予阿魏酸哌嗪溶液50mg·kg-1·d-1灌胃,厄贝沙坦组予厄贝沙坦溶液50mg·kg-1.d-1灌胃,正常组与模型组予以同等体积蒸馏水灌胃,连续8周。监测血糖,8周后处死各组大鼠,行24h尿蛋白定量和肾功能检查,称左肾重,行HE、PAS染色观察肾组织的病理变化,行免疫组化、实时荧光定量PCR观察转化生长因子-β(TGF-β)和纤连蛋白(FN)的表达情况。结果:阿魏酸哌嗪能显著减少糖尿病大鼠肾脏TGF-β和FN的表达,改善肾脏病理变化。结论:阿魏酸哌嗪对糖尿病大鼠肾脏具有保护作用,其作用机制可能与其降低TGF-β和FN的表达有关。

Objective：To study the effect and mechanism of Piperazine Ferulate on STZ- induced diabetic nephropathy in rats. Methods： 32 Sprague - Dawley rats were randomly divided into 4 groups, the normal group, diabetic nephropathy group, Piperazine Ferulate group and Irbesartan group. Piperazine Ferulate group was intragastricly administrated with PF 50 mg·kg^-1·d^-1 ; Irbesartan group was intragastricly administrated with losartan 50 mg·kg^-1·d^-1 ;the normal and diabetic nephropathy groups were intragastricly administrated with identical voluminal distilled water. After 8 weeks, rats from each group were executed. 24 hours urine protein, serum creatinine and the ratio of left renal weight/body weight was measured at the end of the study, renal pathological changes was evaluated and the expression of transforming growth factor - β（TGF- β） and Fibronectin（FN） were examined with immunohistochemisty; The rnRNA of TGF- β and FN were reversely transcribed and quantified by real - time PCR. Results：TGF-β and FiN expressions in Pauperizing Ferulae group and Irbesartan groups were significantly lower than those in diabetic nephropathy group （ P 〈 0.05）. Piperazine Ferulate obviously reduced TGF - β expression and FN deposition in the renal tissues and improved the renal pathological changes in diabetic nephropathy rats. Conclusion： Piperazine Ferulate can evidently relieve renal damage in rats with diabetic nephropathy induced by STZ, which might be related to the down regulation of TGF- β and FN expression.