环糊精饱和水溶液法脱除鹿茸超临界萃取物中胆固醇的研究

Cholesterol Removal from Supercritical Extract of Antler Velvet Using Saturated Aqueous Solution of β-Cyclodextrin

本实验以胆固醇去除率为考察指标,确定了β-环糊精饱和水溶液包合法脱除鹿茸超临界萃取物中胆固醇的最佳工艺,并用HPLC法确定了萃取物中三种有效成分的保留率。实验结果表明,在β-环糊精饱和溶液用量(ml)与萃取物质量(mg)比为1:2、包合温度50℃、包合时间为30min、萃取物的水稀释浓度为0.8%(W/V)条件下,鹿茸超临界萃取物中胆固醇含量可从47622.37μg/g降低到3981.23μg/g,脱除率高达91.64%,尿嘧啶、次黄嘌呤和尿苷三种有效成分保留率分别为77.31%、82.58%、85.19%,从而为鹿茸保健品及药品行业提供了安全卫生的原料基础。

With removal rate of cholesterol as detective index, the optimum conditions of cholesterol removal from supercritical CO2 extract of antler velvet were confirmed using saturated water solution of β-cyclodextrin, and the retention rates of three effective components were determined. The experimental results indicated that the content of cholesterol reduces from 47622.37 μg/g to 3981.23 μg/g, and removal rate of cholesterol is up to 91.64%, while the retention rates of three biological bases are 77.31%,82.58%, and 85.19%, respectively on the conditions of ratio of β-CD to the extract 1：2 （ml/mg）, inclusion temperature 50 ℃, inclusion time 30 min, and diluted concentration of the extract with water 0.8% （W/V）. This process can offer safe and health products by most of cholesterol removal from the extract of antler velvet.