摘要
本实验提出溴甲酚绿-流动注射分光光度法,定量测定可溶性蛋白质含量。在实验确定最优化条件下,检测波长630nm,牛血清白蛋白(BSA)在1.0～280.0mg/L范围内线性关系良好,检测限为0.52mg/L,相对标准偏差为2.26%,实测加标回收率为96.1%～104.2%。该方法分析速度快,操作简便,灵敏度好,能够实现可溶性蛋白的在线检测。
A method was developed for determining soluble protein by flow injection-spectrophotometric analysis with bromocresol green as color developing reagent and 630 nm as detecting wavelengh. Under the optimum conditions, working curve with good linear was obtained. The linear range of BSA is 1.0 ～280 mg/L, the detection limit is 0.52 mg/L, and relative standard deviation （RSD） is 2.26%. The range of recovery rates for different spiked samples is between 96.1% and 104.2%. The characteristics of this method are simple, rapid, and high sensitive and also can be used on-line.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第7期332-335,共4页
Food Science
关键词
流动注射分析
分光光度法
溴甲酚绿
蛋白质
flow injection analysis
spectrophotometry
bromocresol green
protein
