摘要
通过反相离子对高效液相色谱法测定了槟榔及其产品中的生物碱。采用HypersilBDSC18(250×4.6mm,5μm)色谱柱,柱温60℃;流动相为水-甲醇-磷酸-SDS(55ml∶45ml∶0.1ml∶1g),流速为1.0ml/min;采用SPD-20A紫外检测器,检测波长为215nm。结果显示,槟榔碱和槟榔次碱在25min内分离良好。测定的精密度为0.87%～1.58%,加标回收率为96.77%～100.23%。应用该方法对槟榔及其产品中槟榔碱和槟榔次碱的含量进行了测定分析。
A method was developed for quantitative dtermination of arecoline and arecaidine in areca nut and its products by reversed-phase ion-pair high performance liquid chromatography （RP-IP-HPLC）. Chromatographic separation was performed on Hypersil BDS C18 （250×4.6 mm, 5μm） column using a mixture （water： methanol： phosphoric acid： SDS=55 ml ： 45 ml ： 0.1 ml ：1 g） as mobile phase with flow rate 1.0 ml/min and using SPD-20A ultraviolet detection at 215 nm. Arecoline and arecaidine are eluted within 25 min with satisfactory separation. Relative standard deviation of 0.84%～1.56% and average recovery of 96.77%～100.23% for the method are achieved. The contents of alkaloids in different areca nuts and their products were determinded by this method.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第7期345-347,共3页
Food Science
