番茄果皮超临界流体萃取物中类胡萝卜素在大鼠血清中的积累

Accumulation of Natural Lycopene and β-Carotene Extract from Tomato Skin by Supercritical Fluid in Rat Serum

目的:观察一次饲喂萃取物后两种类胡萝卜素在大鼠血清中的积累情况。方法:选雄性SD大鼠80只,随机分为8组,每组10只。每只灌胃1ml萃取物与豆油混合物(5.25mg总类胡萝卜素/ml)。灌胃后于不同时间取一组断头取血,用甲醇和正己依次烷萃取血清中总类胡萝卜素。对萃取物进行C18-HPLC分析。结果:灌胃0.5h后可在血清中检测到番茄红素的信号,3h达到最大值。12h以后,血清中番茄红素的量下降到0.5h的水平。灌胃液中番茄红素浓度/血清中最大番茄红素浓度(3h)为15441:1。β-胡萝卜素具有相似积累规律。

Objective： This study aimed at the observation of carotenoid accumulation in rat serum after single feed of the extract. Method： 80 SD male rats were selected and divided randomly into 8 groups （10 rats/group）. The mixture （1 ml, 5.25 mg total carotenoid/ml） of soybean oil and the extract was administrated by gavage. Blood was collected at fixed time from a group. The total carotenoids were extracted from the serum by methanol and hexane successively. The extracts were analyzed by C18-HPLC-PDA. Results： Serum lycopene is detected after 0.5 h, and its content reaches the maximum after 3 h. After 12 h, serum lycopene content dropps down to the same level as that at the 0.5th h. The ratio of lycopene content by gastric administration to maximum lycopene content （at the 3th h） in serum is 15441 ： 1. The accumulation of β-carotene in serum shows a similar variation.