凉薯种子提取物急性毒性和对KB细胞抑制作用的初步研究

Preliminary Study on Acute Toxicity and Effects of Different Extracts of Pachyrrhizus erosus Urban Seed on KB Cell

目的:观察桂林地区凉薯种子不同组分提取物的体外抗肿瘤作用和对小鼠的急性毒性反应。方法:用MTT法观察凉薯种子的各种提取物对KB细胞的抑制作用;采用一次灌胃后,测定凉薯种子提取物的小鼠半数致死量(LD50)及95%可信限。结果:凉薯种子石油醚提取物的LD50为7.314g/kg(6.328～8.455g/kg),正丁醇提取物的LD50为4.223g/kg(3.586～4.973g/kg);水溶性提取物的最大耐受量大于8g/kg;80μg/ml和40μg/ml的乙酸乙酯提取物对KB细胞72h时的抑制率分别为32.6%(p<0.01)、16.4%(p<0.05);正丁醇提取物80μg/ml对KB细胞72h时的抑制率为49.9%(p<0.01);结论:乙酸乙酯和正丁醇提取物对KB细胞有一定抑制作用;凉薯种子正丁醇提取物和石油醚提取物的毒性较大,水溶性提取物的毒性较小。

Objective： To observe effects of different extracts of Pachyrrhizus erosus Urban （planted in Guilin region） seed on KB cell in vitro and acute toxic reaction in mice after intragastric administrate with the different extracts. Methods： The effects of the extracts on KB cell in vitro were tested by MTT. LD50 and 95% confidence limit were detected after intragastric administration with the different extracts to mice. Results： LD50 and 95% confidence limit of petroleun benzin extract are 7.314 g/kg （6.328～ 8.455 g/kg） respectively, and those of n-butanol extract are 4.223 g/kg （3.586 ～ 4.973 g/kg） respectively. The maximum tolerance of water extract is 8 g/kg. The inhibition rates to KB cell are 32.6% （p〈0.01） and 16.4% （p〈0.05） after 72 hours of the administration with the concentration of 80μg/ml and 40μg/ml of acetoacetate extract respectively. Conclusion： The n-butanol extract and acetoacetate extract show inhibition effect on KB cell, and the toxicity of the nbutanol extract to mice is higher whereas the toxicity of the water extract is lower compared with different groups.