铁调素及铁离子对人成骨细胞(hFOB1．19)内钙离子转运的影响

Influence of hepcidin and iron ion out of osteoblast on calcium transportation

目的研究铁调素（Hepcidin）及铁离子对人成骨细胞（hFOB1．19）内Ca^2＋转运的影响。方法成骨细胞34℃培养3—4天至细胞密度为90％，胰蛋白酶消化后分种于12孔培养板（每孔内置一圆形盖玻片）。（1）空白组加入双蒸水，实验组加入不同浓度Hepcidin（双蒸水配制），干预24h后用激光共聚焦扫描显微镜（cLSM）检测；（2）空白组加入双蒸水，实验组加入不同浓度的DFO和FAC，分别干预后立即用激光共聚焦扫描显微镜检测。结果经激光共聚焦扫描显微镜（CLSM）检测发现：（1）随着成骨细胞外Hepcidin浓度的升高，成骨细胞内钙离子的绿色荧光强度增强；（2）随着成骨细胞外DFO浓度的升高，成骨细胞内钙离子的绿色荧光强度增强；相反，随着成骨细胞外FAC浓度的升高，成骨细胞内钙离子的绿色荧光强度减弱。结论（1）Hepcidin可促进成骨细胞内Ca^2＋增加。（2）成骨细胞外Fe^3＋浓度的减少可以增加细胞内的Ca^2＋，而胞外Fe^3＋浓度增加则减少细胞内的Ca^2＋。

Objective To study the influence of Hepcidin and Fe^3＋ concentrations change out of hFOB 1.19 cells on Ca^2＋ transportation. Methods The hFOB 1.19 was cultured at 34℃ for 3-4 days, being digested with Trypsin, then placed in the 12 hole culture board（one eoverslip in one hole） . （1） Add double distilled water into the control group and Hepcidin of different concentrations into the experimental group. Confocal laser scanning microscope （CLSM） was used in this study 24 hours later ; （2） Add double distilled water into the control group and add DFO and FAC of different concentrations into the experimental group, then confocal laser scanning microscope （CLSM） was used in this study immediately. Results It was observed under CLSM： （1） the fluorescence intensity of Ca^2＋ in hFOB 1.19 increased significantly with the increase of Hepcidin outside them; （2） the fluorescence intensity of Ca^2＋ in hFOB 1.19 also increased gradually with the decrease of Fe^3＋ concentration outside them, however, it decreased with the increase of Fe^3＋ concentration out of them. Condusions （1）The Ca^2＋ transportation was increased with the increase of Hepcidin out of hFOB 1.19 cells ; （2） The Ca^2＋ transportation was increased with the decrease of Fe3＋ out of hFOB 1.19 cells hut was hindered with the increase of Fe^3＋ out of them.