不同因素对三价铬跨Caco-2细胞转运的影响

Effects of Different Factors on Transport of Trivalent Chromium in Caco-2 Cell Monolayers

采用一种体外培养的人小肠上皮细胞模型Caco-2研究了铜、铁、锌、维生素C、蔗糖、草酸钠、乙二胺四乙酸以及柠檬酸钠对三氯化铬和吡啶羧酸铬跨细胞转运的影响,旨在探讨各种因素对不同形式三价铬吸收影响的差异。结果表明:铁显著降低了吡啶羧酸铬和三氯化铬在Caco-2细胞中的转运量(P<0.05),而铜和锌对它们的转运量没有产生显著影响(P>0.05);维生素C、蔗糖、草酸钠、乙二胺四乙酸和柠檬酸钠对吡啶羧酸铬的转运量没有产生显著影响(P>0.05),但维生素C和草酸钠显著增加了三氯化铬在Caco-2细胞中的转运量(P<0.05),蔗糖则显著降低了三氯化铬的转运量(P<0.05)。结果提示三氯化铬相对于吡啶羧酸铬而言,在吸收时更容易受到各种因素的影响。

In order to investigate the effects of various dietary factors on intestinal absorption of different forms of trivalent chromium, a human intestinal epithelial cell model Caco-2 in vitro cultured was used to study the effect of copper, iron, zinc, vitamin C, sucrose, sodium oxalate, EDTA and sodium citrate on transport of chromium chloride and chromium picolinate. The results showed that iron significantly decreased the transport amount of chromium picolinate and chromium chloride in Caco-2 cell monolayers （P〈0.05）. However, copper and zinc produced no significant effect on their transport amount （P〉0.05）. The transport amount of chromium picolinate was not affected by vitamin C, sucrose, sodium citrate, EDTA and sodium oxalate （P〉0.05）. However, the transport amount of chromium chloride was significantly increased by vitamin C and sodium oxalate （P〈0.05）, and decreased by sucrose （P〈0.05）. These results indicated that transport of chromium chloride was more easily affected by various dietary factors than that of chromium picolinate.