应用激光扫描共聚焦显微镜研究卵母细胞和早期胚胎的细胞骨架

Studies of Cytoskeleton in Oocytes and Preimplantation Embryos by Confocal Laser Scanning Microscopy

介绍了应用激光扫描共聚焦显微镜,结合免疫荧光技术研究卵母细胞和早期胚胎细胞骨架的方法。观察了β-微管蛋白、γ-微管蛋白、微丝以及染色体在小鼠卵母细胞和早期胚胎中的分布和形态,讨论了实验过程中的注意事项以及实验结果的分析和处理方法。

This study systematically introduced the method of cytoskeleton detection in mouse oocytes and preimplantation embryos by confocal laser scanning microscopy and immunofluorescent staining technique. We observed the configuration, distribution and localization of β-tubulin, γ-tubulin, microfilaments and chromosomes in mouse oocytes and preimplantation embryos. We also described some key points and tricks of this technique, and discussed analysis method of the experiment results as well.