regⅠ基因表达与肠黏膜损害在急性坏死性胰腺炎中的相关性

Correlation between reg Ⅰ expression and intestinal mucosal lesion of acute necrotizing pancreatitis in rats

目的:研究regⅠ在急性坏死性胰腺炎(acute necrotizing pancreatitis,ANP)大鼠肠组织中的表达,探讨该基因的表达与ANP肠黏膜损害的关系.方法:Sprague-Dawley大鼠随机分为假手术对照组(n=40)和ANP组(n=80).对照组开腹后只翻动胰腺,ANP组大鼠胰胆管恒速逆行注射30g/L牛磺胆酸钠,制成ANP大鼠模型.观察胰腺和小肠的病理改变及小肠黏膜通透性的改变,应用逆转录聚合酶链反应(RT-PCR)检测胰腺、小肠组织中regⅠ mRNA的表达水平,并研究所观察指标与regⅠ基因表达的相关性.结果:ANP组大鼠术后12,24和36h肠黏膜组织病理学评分明显高于对照组(1.8±0.89 vs 0.2±0.42,3.3±1.17 vs 0.3±0.48,4.2±0.95 vs 0.3±0.48,均P<0.01);ANP组大鼠[99mTc]-DTPA排泄率术后12,24和36h较对照组均明显增加(34.70%±4.03% vs 4.62%±1.17%,54.63%±6.94% vs 6.14%±1.42%,66.83%±7.56% vs 7.48%±0.92%,均P<0.01);与对照组相比,ANP组大鼠小肠组织中regⅠ的mRNA水平过度表达,regⅠ的表达水平分别与肠黏膜病理学评分和肠黏膜通透性指数正相关(r=0.6728,0.7092,均P<0.01).结论:ANP时大鼠小肠组织中regⅠ mRNA的表达水平明显上调且与ANP所致肠黏膜损害的严重程度相关.

AIM： To investigate gene expression of Reg Ⅰ in intestinal tissues in rats with acute necrotizing pancreatitis （ANP） and to determine correlation between reg Ⅰ gene expression and intestinal mucosal lesion. METHODS： Sprague-Dawley rats were randomly allocated into the control group （n = 40） and ANP group （n = 80）. The rats in control group received laparotomy only. In ANP group, 30 g/L sodium taurocholate was injected under constant temperature into the pancreatic duct to develop the ANP model. Reverse transcriptase polymerase chain reaction （RT-PCR） was performed to detect reg Ⅰ mRNA level in pancreas and intestines. The pathologic changes of pancreas and intestines were observed, the permeability of intestinal mucosa was estimated.The measured parameters were evaluated to find whether correlation exited between reg Ⅰ mRNA expression level and pathological mucosal changes of ANP. RESULTS： The intestinal pathological scores of ANP rats were signifi cantly higher than that of control group at 12, 24, 36 h （1.8 ± 0.89 vs 0.2 ± 0.42, 3.3 ± 1.17 vs 0.3 ± 0.48, 4.2 ± 0.95 vs 0.3 ± 0.48, all P 〈 0.01）, postoperatively. The excretory rate of [^99mTc]-DTPA in ANP rats were higher at 12, 24, 36 h, postoperatively, compared with control group （34.70% ± 4.03% vs 4.62% ± 1.17%, 54.63% ± 6.94% vs 6.14% ± 1.42%, 66.83% ± 7.56% vs 7.48% ± 0.92%, all P 〈 0.01）. Reg Ⅰ mRNA was highly expressed in intestine in ANP rats compared with that in control group. Reg Ⅰ expression level was positively correlated with intestinal pathological scores（r = 0.6728, P 〈 0.01） and with intestinal permeability （r = 0.7092, P 〈 0.01）. CONCLUSION： Reg Ⅰ mRNA expression is upregulated in intestinal tissues in ANP rats, and expression level of reg Ⅰ is positively correlated with severity of intestinal mucosal lesion caused by ANP.