粉葛试管块根诱导技术的研究

Research on the Induction Technology of the Tuberous Root of Pueraria thomsonii Benth.in the Test Tube

目的建立粉葛无菌苗培养及离体条件下诱导试管块根的体系。方法以粉葛的种子为外植体,MS培养基为基本培养基,培养条件为25℃,2000-3000 Lx光照,12h/d。结果机械破坏种皮可有效地打破种子的休眠。除去顶芽有利于粉葛试管丛生芽的发生。已生根的试管苗转入块根诱导培养基中,根部明显增粗,形成粉葛的试管块根,最佳培养基配方为：MS＋6-BA 2mg/L＋NAA 0.1mg/L＋蔗糖5%。结论无菌离体条件下可以诱导出粉葛试管块根。

Objective To establish the sterilized seedling culture and the test tube earthnut induction system. Methods The seed of Pueraria thornsonii Benth. was cultured in MS culture medium as explant under the light length of 2000 - 3000Lx 12 h/d at 25℃. Results Mechanical break could efficiently smash the dormancy of seed. The shearing away of the top sprout would contribute to the germination of fascicled bud. When the plantlet with induced roots were shifted to tuberous root inducing culture medium, the root obviously augmented which consequently led to the formation of test tube tuberous root. The optimized medium was MS ＋6 - BA2mg/L ＋ NAA 0.1 mg/L ＋ sucrose 5%, Conclusion Test tube tuberous root of Pueraria thornsonii Benth. could be induced in sterile conditions in vitro.