当归注射液对大鼠实验性弥散性血管内凝血血液动力学的影响

Effects of Angelica parenteral Injection on Hemodynamics in Experimental DIC Rats

目的探讨当归注射液(APS)对大鼠实验性弥散性血管内凝血(DIC)血液动力学的影响。方法颈静脉注射Dex-tran 500复制大鼠实验性DIC模型,采用肠系膜微循环观察、器官微区血流和血液流变学测定方法,观察APS对DIC大鼠微血管口径、器官微区血流量、血黏度及血小板功能的影响。结果DIC时,微循环明显障碍,表现为微血管收缩、微血流变慢和血细胞聚集,APS治疗后,微血管明显扩张,其流态和血细胞聚集积分值明显低于NS组(P<0.05);APS和NS组均能改善胃、肠、肝的微区血流量,但前者效果优于NS(P<0.05);APS治疗后,血黏度、血小板黏附率和聚集率明显低于NS治疗组,且红细胞变形能力明显增强(P<0.05)。结论APS通过扩张微血管、增加器官血流量、降低血粘度和抑制血小板聚集作用,干预DIC的转归。

Objective To explore the effects of Angelica parenteral Injection （ APS ） on hemodynamics in rats with disseminated intravascular coagulation （DIC）. Methods A model of DIC was made in rats by Dextran500 injection from jugular vein, and effects of APS on micro - vessels, micro - area blood flow volume of organs, blood viscosity and platelet aggregation were studied in rats by an observation of mesenteric microcirculation, determination of hemorheology and blood flow volume. Results The microcirculation disturbance was mainly micro - vessels contraction, slow micro - blood flow and micro - thrombus after Dextran 500 injection. The result showed that APS could dilate micro - vessel obviously and reduce multiple score values of blood flow and blood cell aggregation, there were obvious differences compared with normal saline （NS） group （ P 〈 0.05 ）. The micro - area blood flow volume of stomach, intestine and liver was evidently increased in APS and NS groups, however, the former effect was superior to NS group （ P 〈 0.05 ）. After treatment with APS, the blood viscosity and platelet aggregation were obviously lower than those of NS group, and meantime the deformity ability of RBC was obviously increased （ P 〈 0.05 ）. Conclusion APS can influence the recovery, of DIC through dilating micro - vessel, increasing blood flow volume of organs, and reducing blood viscosity and platelet aggregation.