毛细管气相色谱法测定雪抑乐中冰片的含量

Content Determination of Bornol in Xueyile by Capillary GC

目的建立毛细管气相色谱法测定雪抑乐中冰片的含量。方法色谱柱为SPBTM-1 Capillary Column(30m×0.25mm×0.25μm);FID检测器;柱温100℃;进样器温度235℃;检测器温度245℃;载气N2;流速1.2ml·min-1;分流比1∶10;内标萘。结果毛细管气相色谱法测定冰片的分离度和线性良好,龙脑平均回收率为96.44%(RSD=0.90%,n=6),异龙脑平均回收率为96.78%(RSD=1.15%,n=6)。结论该方法简便、快速、准确,可用于雪抑乐质量控制。

Objective To establish a Capillary GC method for the determination of Bomol in Xueyile. Methods Chromatographic conditions were SPBTM - 1 Capillary Column （ 30 m × 0.25 mm ×0.25 μm）, Detector： FID, Column temperature ： 100 ℃, injector temperature ： 235 ℃, detector temperature ： 245 ℃, Carrier gas ： N2, Flow rate ： 1.2 ml ·min^ - 1, Split ratio 1 ： 10, and Internal standard ： naphthalene. Results The isolation and the linearity were fine with the recovery rate of homo1 96.44% （ RSD = 0.90%, n = 6）, isobomol 96.78% （RSD = 1.15 %, n = 6 ）. Conclusion The method is accurate, rapid and simple. It can be applied for the quality control of homol.