反相高效液相色谱法测定不同地区不同采集期茜草中茜草素的含量

Determination of Mcilugin in Radixrubiae in Different Resources and Different Collect Time by PR-HPLC

目的采用反相高效液相色谱（RP-HPLC）法测定不同地区、不同采集期茜草中大叶茜草素的含量。方法色谱柱Lichmsorb C18柱（150mm×4．6mm，5μm）；紫外检测波长250nm；流动相为甲醇-0．1％磷酸水溶液（85：15），流速0．8ml·min^-1，柱温为35℃。结果大叶茜草素在0．021～0．105μg具有良好的线性关系，r=0．9995，平均回收率为99．82％，RSD为0．85％（n=5）。结论该方法简便快捷、精密度高、结果准确。

Objective To develop an RP - HPLC method for determination of the contents of mcilugin in radixrubiae of the different resources and different collect times. Methods The extracts were seperated on Liehrosorb C18 （150 mm × 4.6 mm ,5 μm） column with methanol-0.1% phosphoric acid （85：15） as mobile phase. The flow rate was 0.8 ml · min^-1. The detection wavelength was at 250 nm. Column temperture was 35℃. Results The method was linear within the range of 0. 021 -0. 105 μg （r = 0.999 5 ）. The average recoveries of assay were 99.82% and RSD 0, 85% （ n = 5 ）. Conclusion The method is simple, rapid and accurate.