口服当归补血汤后阿魏酸在大鼠体内的组织分布(英文)

Tissue Distribution of Ferulic Acid after Oral Administration of Tangkuei Blood-Supplementing Decoction to Rats

目的研究大鼠灌胃给予当归补血汤后阿魏酸在体内的组织分布特性。方法采用高效液相色谱法；色谱柱为Kromasil C18（4．6mm×150mm，5μm）；流动相为0．2％甲醇乙腈-0．2％磷酸0．2％三乙胺水溶液（18：82）；检测波长322nm；柱温40％；流速0．8ml／min。结果各组织中的阿魏酸在10～400ng范围内与峰面积呈良好的线性关系；回收率平均为87．97％±1．95％；日内和日间的RSD分别1．92％～3．87％和3．21％～6．39％之间。阿魏酸在各组织器官中浓度差异较大。结论大鼠灌胃给予当归补血汤后阿魏酸主要在肝脏和。肾脏分布，阿魏酸不能通过血脑屏障。

Objective To investigate the tissue distribution of ferulic acid in rats after oral administration of Tangkuei blood - supplementing decoction. Methods HPLC was used ; Column ： Kromasil C18 （ 150mm × 4.6mm , 5 μm ） ; mobile phase ： 0.2 % methanol acetonitrile - 0.2 % phosphoric acid 0.2 % triethylamine solution （ 18 ： 82 ） ; detection wavelength was at 322nm; temperature was at 40℃ ; flow velocity was 0.8ml/min. Results The linear ranges of ferulic acid in the heart, liver, spleen, lung and kidney were 10 -400ng. The recovery of ferulic acid was 87.97% ± 1.95% on average . The intra - day and inter - day RSD were 1.92% - 3.87% and 3.21% 6.39% , respectively. The concentrations of ferulic acid were very different in organs. Conclusion The major distribution organs of ferulic acid in rats are liver and kidney. Ferulic acid can not pass though the blood -barrier.