金雀异黄素抑制SGC7901细胞增殖、迁移和粘附的研究

The Effect of Genistein on Proliferation,Migration and Adhesion of Human Gastric Adenocarcinoma SGC7901 Cell

目的探讨金雀异黄素(Gen)对人胃腺癌SGC7901细胞增殖、迁移以及与血管内皮细胞粘附的影响。方法利用细胞计数和MTT方法检测Gen对SGC7901细胞增殖的影响;利用细胞划痕实验检测Gen对SGC7901细胞迁移的影响;在单层脐静脉内皮细胞上加入SGC7901细胞,检测Gen对SGC7901细胞与血管内皮细胞粘附的影响;利用免疫荧光实验观察Gen对SGC7901细胞中E-cadherin、β-catenin和肌动蛋白(actin)的分布的影响;利用Western blotting检测Gen对SGC7901细胞中FAK、p-FAK、E-cadherin、β-catenin蛋白表达的影响。结果Gen可以抑制SGC7901细胞的增殖、迁移和粘附,影响SGC7901细胞中E-cadherin、β-catenin和actin的分布,下调p-FAK和E-cadherin的表达。结论Gen抑制SGC7901细胞的增殖、迁移和与血管内皮细胞粘附,可能是通过诱导细胞中E-cadherin、β-catenin和actin的重排、下调p-FAK和E-cadherin的表达等实现的。

Objective To evaluate the effect of genistein （Gen） on proliferation, migration and adhesion to endothelium of human gastric adenocarcinoma SGC7901 cell. Methods Cell counting and 3-（4, 5-dimethylthiazol-2-yl）-2, 5-diphenylterazolium （MTT） assays were performed to study the effect of Gen on SGC7901cell proliferation, and wound-healing assay was used to test the effect of Gen on SGC7901 cell migration, and SGC7901 cells adding to a monolayer of ECV304 cells was used to explore the ability to adhere to endothelium. Immunofluorescence assay was used to e- valuate the effect of Gen on distribution of E-eadherin, β-eatenin, and actin, and the Western blotting assay was used to test the expression of FAK.p-FAK.E-eadherin and β-eatenin in SGC7901 cell. Results Gen suppressed SGC7901 cell proliferation, migration, and SGC7901 cell-endothelial cell adhesion, and modulated the distribution of E-eadherin, β- eatenin, and actin cytoskeleton, and down-regulated the expression of p-FAK and E-eadherin in SGC7901 cells. Conclusion Inhibition of SGC7901 cell proliferation, migration, and adhesion on endothelium by Gen might be modulated through its regulating the redistribution of E-eadherin, β-catenin, and actin cytoskeleton, and down-regulating the expression of p-FAK and E-eadherin.