紫花地丁抗炎及体外抑菌作用活性部位的筛选研究

Study on Bioactive Parts with Anti-inflammatory Effects and Anti-bacteria Function in Viola Yedoensis Makino

目的:筛选出紫花地丁抗炎及抑菌作用的活性部位。方法:通过二甲苯致小鼠耳肿胀实验及体外抑菌实验,观察紫花地丁水煎剂、紫花地丁乙醇提取物各分离部位对炎症的影响及体外抑菌作用。结果:紫花地丁水煎剂、紫花地丁乙醇提取物乙酸乙酯部位对二甲苯所致的小鼠耳肿胀有明显的抑制作用(P<0.05),对大肠杆菌、沙门氏菌、金色葡萄球菌、表皮葡萄球菌有较强的抑菌作用。结论:紫花地丁水煎剂、紫花地丁乙醇提取物乙酸乙酯部位是紫花地丁发挥抗炎、抑菌作用的主要活性部位。

Objective： To identify the bioacfive parts with anti -inflammatory effects and anti -bacteria function in Viola yedoen- sis Makino. Methods： The models of dimethylbenzene - induced mouse ear edema and vitro antibacterial experiment were performed. Results： The water extract and ethyl acetate part could inhibit ear edema （ P 〈 0. 05） and had antibacterial activities agaimt Eseheriehia coLi, Salmonella, Staphylococcus aureus, Staphylococcus epidermidis. Conclusion： The water extract and ethyl acetate part are the main bioacfive parts of Viola yedoeusis Makino.