β-catenin在角化性皮肤病与非黑素细胞性皮肤肿瘤中的表达

Expression of β-catenin in keratosic dermatosis and non-melanocytic skin tumors

目的研究β-catenin在角化性皮肤病和非黑素细胞性皮肤肿瘤中的表达及意义。方法应用免疫组化EnVision二步法检测β-catenin蛋白在10例脂溢性角化病(SK)、26例光线性角化病(AK)、17例Bowen病(BD)和35例初发皮肤鳞状细胞癌(SCC)皮肤组织中的表达,以10例正常皮肤组织作为对照组。结果SK、AK、BD和SCC皮肤组织中β-catenin的表达显著高于对照组皮肤组织(P<0.001)。β-catenin的细胞质和(或)细胞核表达在SK与AK组间无明显差异(P>0.05),但均显著低于BD组(P<0.01)。BD组β-catenin表达缺失显著低于SCC组(P<0.05)。SCCⅠ级与Ⅱ、Ⅲ级相比,β-catenin的细胞质和(或)细胞核表达及表达缺失均无显著性差异(P>0.05)。结论在正常皮肤向角化性皮肤病和非黑素细胞性皮肤肿瘤的进展过程中,细胞质和(或)细胞核中β-catenin的异常表达可能起了一定作用。

Objective skin tumors. Methods To study the expression and significance of β-catenin in keratosic dermatosis and non-melanocytic β-catenin expression was determined in 10 specimens of normal skin, 10 cases of seborrheic keratosis（ SK）, 26 cases of actinic keratosis （AK）, 17 cases of Bowen＇s disease（BD） and 35 cases of primary cutaneous squamous cell carcinoma（SCC） by immunohistochemical EnVision two-step staining method. Results The expression of β-catenin was significantly higher in SK, AK, BD and SCC than in normal skin （P 〈 0.001）. There was no significant difference in the cytoplasmic and/or nuclear expression of β-catenin between SK and AK （ P 〉 0.05） , but that was significantly lower in SK and AK than in BD（ P 〈0.01 ）. Loss of β-catenin expression was significantly lower in BD than in SCC （P 〈0.05）. There was no significant difference in cytoplasmic and/or nuclear expression of β-catenin and loss of β-catenin expression between grade I and grade 11 and m in SCC （P 〉0.05）. Conclusion The cytoplasmic and/or nuclear expression of β-eatenin may play a role in the progression from normal skin to keratosie dermatosis and nonmelanocytic skin tumors.