胃腺癌组织中微淋巴管密度的检测及其临床病理意义

Detection of Lymphatic Microvessel Density in Primary Gastric Adenocarcinoma and Its Clinicopathological Significance

目的探讨原发性胃腺癌中微淋巴管密度的临床病理意义。方法应用单克隆抗体D2-40检测原发性胃腺癌组织周边区和中心区及其正常胃组织中微淋巴管密度(LMVD),分析其与淋巴管浸润、淋巴结转移及其他临床病理参数之间的关系。结果胃腺癌周边区LMVD明显高于中心区LMVD(P<0.05)和正常胃组织LMVD(P<0.05),且淋巴管为功能性的。周边区LMVD与肿瘤的Ming分型、淋巴管浸润及淋巴结转移密切相关(P<0.05),而与患者的年龄、性别、肿瘤的分化程度及浸润程度无关(P>0.05)。中心区LMVD与这些临床病理参数之间均无相关性(P>0.05)。结论原发性胃腺癌癌周LMVD和淋巴管浸润、淋巴结转移密切相关,检测癌周LMVD是预测胃癌淋巴结转移的有效指标之一,有助于判断胃癌的预后。

Objective To investigate the clinicopathological significance of the lymphatic microvessel density （LMVD） in primary gastric adenocarcinoma. Methods Monoclonal antibody D2-40 was used immunohistochemically to detect the lymphatic microvessel density（LMVD） in the intra- and peri-tumoral areas of the primary gastric adenocarcinoma and normal gastric tissue, and analysis the relationship among the lymphatic microvessel density（LMVD）, lymphatic invasion, lymph node metastasis and other clinicopathological parameters of the primary gastric adenocarcinoma. Results LMVD of the peri-tumoral area was significantly higher than that of intra-tumoral area and nomal tissue（P〈0. 05）. LMVD of the peritumoral area was significantly associated with Ming Classification, lymphatic invasion and lymph node metastasis（P〈0. 05）, while less associated with age, sex, differentiation and invasion of the tumor（P〉0. 05）. LMVD of the intra-tumoral area was not correlated with these clinicopathological parameters（P〉0. 05）. Conclusion LMVD of the peri-tumoral area was significantly associated with lymphatic invasion and lymph node metastasis, and detection of LMVD in perbtumoral area can be used for prediction of lymph node metastasis.