摘要
AIM： To study the immunoregulatory effect of 1,25-dihydroxyvitamin-D3 Von dominant Thl response in rats. METHODS： Sixty adult Lewis rats were randomized into three groups. Rats in group 1 （n=25） were treated with 1,25-（OH）2D3 first and then challenged with LPS, rats in group 2 （n=25） were treated with vehicle first and then challenged with LPS. Ten animals in groups 1 and 2 were preserved for mortality observation. The remaining animals were injected （i.p） with endotoxin, 24 h after the last administration of 1,25-（OH）2D3 and vehicle. Rats in group 3 （n=10） were treated with 1,25-（OH）2D3 only. Serum IL-12, IFN-y, IL-2 and IL-4 levels were measured and target gene of 1,25-（OH）2D3 on Th cells was studied after 6 h. Gene abundance was verified by real-time quantitative PCR. RESULTS： No death occurred in rats pretreated with 1,25-（OH）2D3 after LPS injection. Death occurred 9 h after LPS injection in rats pretreated with the vehicle, and the number of deaths was 5 within 24 h, with a mortality rate of 50%. There was no change in the number of deaths within 96 h. Six hours after endotoxin stimulation, serum IL-12 and IFN-y levels decreased significantly in rats pretreated with 1,25-（OH）2D3 as compared with those in rats pretreated with the vehicle. The serum content of these two cytokines was very low in rats not challenged by endotoxin, and there was a significant difference as compared with the previous two groups. CONCLUSION： 1,25-（OH）2D3 attenuates injuryinduced by the lethal dose of 1PS, regulates Thl and Th2 cells at the transcription level, and dominantly responds to cytokine production in rats.
AIM: To study the immunoregulatory effect of 1,25-dihydroxyvitamin-D3 Von dominant Th1 response in rats. METHODS: Sixty adult Lewis rats were randomized into three groups. Rats in group 1 (n=25) were treated with 1,25-(OH)2D3 fi rst and then challenged with LPS,rats in group 2 (n=25) were treated with vehicle fi rst and then challenged with LPS. Ten animals in groups 1 and 2 were preserved for mortality observation. The remaining animals were injected (i.p) with endotoxin,24 h after the last administration of 1,25-(OH)2D3 and vehicle. Rats in group 3 (n =10) were treated with 1,25-(OH)2D3 only. Serum IL-12,IFN-γ,IL-2 and IL-4 levels were measured and target gene of 1,25-(OH)2D3 on Th cells was studied after 6 h. Gene abundance was verifi ed by real-time quantitative PCR. RESULTS: No death occurred in rats pretreated with 1,25-(OH)2D3 after LPS injection. Death occurred 9 h after LPS injection in rats pretreated with the vehicle,and the number of deaths was 5 within 24 h,with a mortality rate of 50%. There was no change in the number of deaths within 96 h. Six hours after endotoxin stimulation,serum IL-12 and IFN-γ levels decreased signifi cantly in rats pretreated with 1,25-(OH)2D3 as compared with those in rats pretreated with the vehicle. The serum content of these two cytokines was very low in rats not challenged by endotoxin,and there was a signifi cant difference as compared with the previous two groups. CONCLUSION: 1,25-(OH)2D3 attenuates injuryinduced by the lethal dose of LPS,regulates Th1 and Th2 cells at the transcription level,and dominantly responds to cytokine production in rats.
基金
National Basic Research Program of China,2003CB515502
