中药红藤方对大鼠的异位子宫内膜芳香化酶表达的影响

Effect of "Caulis Sargentodoxae Formula" on Aromatase Expression in Endometrial Rats

目的:探讨中药红藤方对异位子宫内膜的抑制作用。方法:用手术自体移植法成功建立的子宫内膜异位症大鼠模型,随机分成5组:红藤方高剂量组(80g生药/kg,A组)、低剂量组(20g生药/kg,B组)、达那唑组(60mg/kg,C组)、去势组(D组)和模型组(E组),连续给药21d后,检测各组的血清E2水平及异位内膜和卵巢的芳香化酶的变化;另测定异位内膜的体积。结果:A组、C组和D组的异位内膜生长均明显受到抑制,且3组间无统计学差异(P>0.05),与E组比有统计学差异(P<0.05);组织学观察可见,异位内膜呈退化趋势,A、C和D组间大鼠血清中的E2水平无统计学差异(P>0.05),与E组相比有统计学差异(P<0.05);A组、C组及D组异位内膜、卵巢组织的芳香化酶P450低表达,A组与E组间有统计学差异(P<0.05)。结论:红藤方可通过抑制异位内膜、卵巢的芳香化酶活性表达,降低局部雌激素含量,抑制异位内膜的生长,使异位内膜萎缩。

Objective： To explore the inhibit effect of ＂Caulis Sargentodoxae Formula＂ on the ectopic endometrium. Methods： The model of endometriosis was established by Autotransplantion, the animals were divided randomly into 5 groups： ＂Caulis Sargentodoxae Formula ＂ high dose group （80 g/kg, Group A）, low dose group （20 g/kg, Group B） and danazol group （60 mg/kg, group C）, castrate dose group （group D）, control group （group E）. After continuous treatment for 21 d, the level of E2, aromatase in ectopic endometrium and ovary were detected for each group, the volume of the ectopic endometrium was also measured. Results： The growth of endometrium was obviously inhibited in group A, C and D. The inhibitory rates were not significantly different between these groups （P〉0.05）, but were significantly different compared with group E （P〈0.05）. Observation from the morphology features showed that implanted endometrial become degenerating. The level of E2 was not significantly different between group A, C and group D, but were significantly different compared with group E （P〈0.05）. And also there was a significantly decrease in the expression of aromatase of ectopic endometrium and ovary in group A, C and D （P〉0.05）, however, there was a significant difference between group A and group E （P〈0.05）. Conclusion： ＂Caulis Sargentodoxae Formula＂ can hold-back the expression of aromatase in ectopic endometrium and ovary so as to decrease the level of E2, it can inhibit the growth of the ectopic endometrium.