II型核受体PPARγ和金属蛋白酶MMP-2、MMP-9在早孕胎盘组织中的表达及意义

Expression of II Typical Nucli Receptor of PPARγ and MMP-2, MMP-9 in the Placenta Tissue from First Trimester of Pregnancy

目的:探讨II型核受体的过氧化物酶体增殖物激活型受体γ(PPARγ)、金属蛋白酶(MMP)-2和MMP-9与细胞滋养细胞浸润的关系。方法:采用免疫组织化学、实时RT-PCR和免疫印迹技术检测早孕早期(孕6-8周,A组)和早孕晚期(孕11-12周,B组)绒毛组织中PPARγ、MMP-2和MMP-9mRNA及蛋白的表达。结果:PPARγ蛋白主要定位在早孕绒毛细胞滋养细胞核及绒毛外滋养细胞柱和细胞岛的细胞滋养细胞核内,MMP-2和MMP-9蛋白阳性颗粒主要存在于绒毛细胞滋养细胞和合体细胞滋养细胞胞浆内,绒毛间质细胞内亦有阳性染色,且A组绒毛PPARγ表达量高于B组(P<0.05),而MMP-2和MMP-9表达量在B组高于A组(P<0.05),与PPARγ呈负相关(r=-0.74,-0.68,P<0.01);且在A组MMP-2表达量多于MMP-9,而B组则MMP-9多于MMP-2,但无显著性差异。结论:PPARγ在调节滋养细胞浸润过程中起重要作用,而且其作用可能是通过调节MMP-2和MMP-9的表达实现的,为深入探讨PPARγ及其配体在滋养细胞浸润机制中的作用提供实验基础。

Objective： To investigate the relationship of PPARγ, MMP-2 and MMP-9 with trophoblast invasion, Methods： The expressions of the protein and mRNA of PPARγ, MMP-2 and MMP-9 were detected in trophoblast of early （week 6-8, group A） and late （week 11-12, group B） first trimester of pregnancy by immunohistochemistry, Real Time RT-PCR and Western blotting technique. Results： PPARγ protein was located mainly in the nuclei of cytotrophoblasts from proximal and distal columns of the anchoring villi and villous cytotrophoblast MMP-2 and MMP-9 proteins were located mainly in the intracytoplasm of cytotrophoblast and syncytiocytotrophoblast, as well as the villous stromal core. The expression level of PPARγ was higher in group A than that in group B （P〈0.05）, and the expressions of MMP-2 and MMP-9 were more in group B than that in group A （P〈0.05）, there was negative correlation between PPARγ and MMP-2, MMP-9 （r = -0.74, -0.68, P〈0.01）. Conclusion： PPARγ plays an important role in the modulation of thophoblast invasion, PPARγ ligands can inhibit invasion through down-regulating MMP-2 and MMP-9 expressions, these provide experiment basement for deeply investigation of PPARγ ligands in the mechanism of cytotrophoblast invasion.