骨桥蛋白mRNA在口腔鳞癌与正常黏膜中的表达及临床意义

Expression of osteopontin mRNA in oral squamous cell carcinoma and normal oral mucosa

目的探讨骨桥蛋白(OPN)mRNA在口腔鳞状细胞癌(OSCC)及配对正常口腔黏膜组织中的表达及临床意义。方法用SYBR Green I荧光定量RT-PCR方法检测30例OSCC患者肿瘤组织及其配对的正常口腔粘膜中OPN mRNA的表达。结果Real time RT-PCR检测结果表明,OPN mRNA在OSCC和配对正常粘膜的表达水平分别为4.17±0.51和0.97±0.12,上调4.3倍(P<0.001)。在30例OSCC标本中,OPN mRNA在高分化鳞癌中的表达为2.16±0.17,中/低分化鳞状细胞癌中的表达为6.80±0.72,下调3.1倍,P<0.05;在颈淋巴结阳性组表达为6.38±0.56,颈淋巴结阴性组为2.89±0.32,上调2.2倍,P<0.05;在临床早期表达为2.34±0.17,临床晚期表达为4.73±0.35,下调2.0倍,P<0.05。结论在OSCC的癌变过程中OPN基因的表达水平显著上调,可能是OSCC诊断、治疗及预后判断的一个靶点基因;OPN在OSCC分类诊断中有应用价值。

Objective To investigate osteopontin （OPN） mRNA expression in oral squamous cell carcinoma （OSCC） and normal oral mucosa tissues. Methods Differential OPN gene expression were detected in 30 cancerous tissues and their paired normal tissues using real-time reverse transcription-PCR （real-time RT-PCR）, and the data were analyzed statistically. Results Real-time RT-PCR results demonstrated that the relative expression level of OPN mRNA in the cancerous tissues were significantly higher than that in paired normal samples （4.17±0.51 vs 0.97±0.12, P〈0.001）, showing a 4.3 -fold up-regulation. In the 30 OSCC specimens, OPN mRNA expression in the OSCC of histological grades I showed a 3.1-fold down-regulation, significantly lower than the expression in grade Ⅱ/Ⅲ tumors （2.16±0.17 vs 6.80±0.72, P〈0.05）; its expression was significantly lower in early stage than in advanced stage OSCCs （2.34±0.17 vs 4.73±0.35, P〈0.05）. In cases of cervical lymph node metastasis, the expression was significantly higher than that in cases without lymphatic metastasis （6.38±0.56 vs 2.89±0.32, P〈0.05）. Conclusion OPN mRNA overexpression may play an important role in OSCC carcinogenesis and can be a potential target for OSCC therapy.