胆汁损伤胃黏膜的实验研究

Role of bile in rat gastric mucosal injury due to duodenogastric reflux

目的通过大鼠实验模型探讨胆汁对胃黏膜的损伤作用。方法SD大鼠分成4组:十二指肠胃反流(DGR)组、DGR+胆管结扎(BDL)组、BDL组、对照组。术后12周处死大鼠,观察胃黏膜损害及病理组织学改变,电子显微镜硝酸镧标记观察胃黏膜细胞间的紧密连接。结果DGR组比去除胆汁的DGR,出现胃黏膜的充血、小凹超常增生和黏膜细胞间的紧密连接受损程度严重。结论在十二指肠胃反流所致胃黏膜的损伤中,去除胆汁可使胃黏膜的损伤减轻,胆汁是胃黏膜损伤的重要因素。

Objective To explore the effect of bile in inducing gastric mucosal injury in rats. Methods SD rats were divided into 4 groups, namely bile duct ligation group, duodenogastric reflux （DGR） group, DGR plus bile duct ligation group and normal control group. The pathological changes in the gastric mucosa and tight junction 3 months after gastrojejunostomy were observed and compared with the findings in the normal control rats. Results Compared with the rats in DGR plus bile duct ligation group, the rats in DGR group showed obvious gastric mucosal hyperemia, foveolar hyperplasia and severely impaired tight junction between the gastric mucosal cells. Conclusion Bile plays an important role in gastric mucosal injury due to DGR.