匹罗卡品致痫大鼠脑内NF-κB及COX-2的表达

Expression of NF-κB and COX-2 in hippocampus of the seizure rat induced by pilocarpine

为探讨癫痫大鼠脑内核转录因子NF-κB及炎性因子环氧化酶-2(COX-2)的表达,本研究采用匹罗卡品(30mg/kg)诱导大鼠癫痫发作后,通过免疫组织化学和Westernblot方法,对癫痫大鼠脑内,主要是海马NF-κB和COX-2的表达进行了观察。结果显示:匹罗卡品诱导大鼠癫痫发作后,大鼠脑内尤其是海马神经元的胞浆和胞核内NF-κBp65免疫反应阳性增强,核移位增多,蛋白产物表达增多;COX-2免疫反应阳性明显增强,蛋白产物表达增多。以上结果提示,癫痫发作后可引起核转录因子NF-κB的活化及炎性因子COX-2的高表达。

To investigate the expressions of NF-κB and cyclooxygenase-2 （COX-2） in the brain of the seizure rats, we applied the method of the immunocytochemical staining （ICC） and Western blot analysis to detect the expression of NF-κBp65 and COX-2 in the region of the hippocampus in the seizure rats induced by pilocarpine （30 mg/kg）. The results showed that both the immunoreactivity and the expression of NF-κBp65 and COX-2 obviously enhanced and the nuclear shif of the NF-κB p65 in the neurons in the hippocampus region increased. These findings suggest that the seizure may increase the expressions of the activated nuclear factor NF-κB and COX-2 in the brain.