Hedgehog信号蛋白在胰腺癌中的表达及临床意义

Expression of hedgehog signal molecules in pancreatic cancer and their correlation with clinical and pathologic characteristics

目的 研究Hedgehog相关信号蛋白Ihh、Ptc和Smo在胰腺癌中的表达情况及其临床意义。方法 采用免疫组织化学方法检测54例原发性胰腺癌及5例正常胰腺石蜡标本中Hedgehog信号蛋白（Ihh、Ptc、Smo）的表达情况，并统计分析其阳性率与临床病理特征的相关性；采用Westernblot比较21例新鲜胰腺癌及癌旁胰腺组织标本Hedgehog信号蛋白表达量的差异。结果 54例胰腺癌标本中，Ihh、Ptc及Smo阳性率分别为70．4％、64．8％和88、9％，5例正常胰腺组织中均无阳性表达；Ptc阳性率与肿瘤大小、分化程度、淋巴结转移情况及肿瘤TNM分期显著相关，Smo表达率则与肿瘤分化程度显著相关（P〈O．05）；21例新鲜胰腺癌标本中Ihh、Ptc、Smo平均表达量均显著高于癌旁胰腺组织（P〈O．05）。结论 胰腺癌组织中Hedgehog信号蛋白表达量显著增加；Hedgehog信号蛋白与胰腺癌临床病理特征相关。

Objective To investigate the expression of hedgehog signal molecules （Ihh,Ptc and Smo） in human pancreatic cancer and their correlation with clinical and pathologic characteristics. Methods Fifty-four pancreatic cancer samples and 5 normal pancreatic tissues were examined by im- munohistochemistry for the expression and localization of hedgehog signal molecules （Ihh, Ptc and Smo）. Meanwhile,correlation of hedgehog signal molecules to clinical and pathologic characteristics of pancreatic cancer was analyzed. Western blot was carried out to analyze the expression of Ihh,Ptc and Smo in 21 freshly removed pancreatic cancer tissues and paraneoplastic pancreatic tissues. Results Of the 54 pancreatic cancer samples, immunoreactivity of Ihh, Ptc and Smo was observed in 70. 4%, 64.8% and 88.9% of the cancer tissues, respectively. No immunoreactivity was observed in the ductal cells of all the 5 normal pancreas tissues. Expression of Ptc was strongly correlated with tumor size, differentiation grade of tumor cells,lymph node metastasis and TNM stage of tumor,and Smo expression strongly correlated with the differentiated grade of tumor cells （P〈0.05, respectively）. Western blot analysis revealed that the expression of Ihh, Ptc and Smo was significantly higher in pancreatic cancer compared to paraneoplastic pancreatic tissues （P〈 0. 05）. Conclusion The expression of Hedgehog signal molecules is significantly higher in pancreatic cancer tissues. These molecules are correlated to clinical and pathologic characteristics of pancreatic cancer.