肿瘤相关巨噬细胞对非小细胞肺癌MCP-1、MIP-1的影响

Influence of TAMs on monocyte chemoattractant protein-1 and macrophage inflammatory protein-1 in NSCLC

目的观察肿瘤相关巨噬细胞（TAMs）U937和非小细胞肺癌细胞（NSCLC）A549在体外不同的共培养条件下，对单核细胞趋化蛋白-1（MCP-1）、巨噬细胞炎性蛋白。1（MIP-1）表达的影响。方法采用jeremy培养方法．分为三组：1组用不同浓度的U937细胞与A549细胞共培养24h；2组同浓度的U937细胞与A549细胞共培养不同时间；3组用A549在不同时间段单独培养，采用原位杂交方法分别检测三组A549细胞中MCP-1、MIP-1mRNA表达情况。结果随着U937细胞浓度逐渐增高以及共培养时间的逐渐延长．癌细胞A549中MCP-1、MIP-1mRNA表达的阳性系数均值也相应地增高，明显高于U937细胞浓度为0×10^5／ml的共培养组以及各时段对照组（均P〈0．05）。结论TAMs细胞U937和NSCLC细胞A549的相互作用，与A549细胞MCP-1，MIP-1mRNA的阳性表达密切相关，并存在着浓度和时问的依从性。

Objective To observe cocultured TAMs cell U937 and NSCLC cell A549 effecting on MCP-1, MIP-1 expression under different conditions in vitro. Methods The cell U937 of different concentrations（0,1,2,4,8 × 10^5/ml） was respectively selected to coculture with the cell A549 of certain concentration（ 1.0 × 10^5/ml） for 24 hours,in situ hybridization was carried out to detect MCP-1, MIP-1mRNA positive expressions in cell A549 of every concentration group. Under the condition that both concentrations o{ cell A549 and cell U937 are 1.0 × 10^5/ml, the same method was used to measure MCP-1, MIP-1mRNA positive expressions in cell A549 in different periods（4h, 8h, 16h,32h,48h） .Results The positive expression ratios of MCP-1 ,MIP-1mRNA in cell A549 significantly increased along with addition of the cell U937 concentration and delay of the cocultural time, apparently higher than that of pri- mary cocultured group that the cell U937 was 0× 10^5/ml and that of contrast group at every period（all P 〈 0.05 ）. Conclusion The mutual interaction between TAMs and NSCLC cells has closely positive correlation with MCP-1, MIP-lmRNA positive expressions in cell A549 ,and depends on cocultural concentration and time.