磷脂酶A2受体基因多态性与缺血性脑卒中关系的研究

A population-based study of phospholipase A2 recepter gene for ischemic stroke in a Chinese population

目的探讨磷脂酶A2受体基因多态性与缺血性脑卒中的关系。方法以rs2203053和rs4665135位点为遗传标记,应用多聚酶链-限制性片段长度多态性(PCR-RFLP)技术检测643例缺血性脑卒中患者和404例健康对照人群的磷脂酶A2受体基因型。结果缺血性脑卒中组与对照组rs2203053和rs4665135位点的等位基因、基因型频率差异无显著性。结论磷脂酶A2受体基因与缺血性脑卒中的发病可能无关。

Objective To investigate the genetic association between the PLA2R gene and ischemic stroke. Methotis 643 patients with ischemic stroke were recruited into this study, and 404 healthy people were as controls. SNP rs2203053,a C to T base change located in intron 3,and rs4665135,a C to T base change located in intron 9 of the gene, were used as genetic markers. PCR-based restriction fragment length polymorphism analysis was applied to genotype rs2203053 and rs4665135 （Bstn I and Hae Ⅲ site）. Results The frequency of allele C, and genotype CC, CT and T1p showed no association between cases and controls. Conclusion The present study suggests that the PLA2R gene is unlikely to contribute to the etiology of ischemic stroke in Chinese.