罗格列酮对2型糖尿病大鼠模型心肌缺血再灌注损伤的保护作用

The protective effects of rosiglitazone on myocardial ischemia-reperfusion injury in type 2 diabetic rats

目的观察罗格列酮对2型糖尿病大鼠模型心肌缺血再灌注损伤的保护作用。方法高脂饮食联合小剂量链脲菌素(STZ)复制大鼠2型糖尿病模型;结扎大鼠左冠状动脉前降支30min,再灌注120min,建立大鼠心肌缺血再灌注(I/R)损伤模型。将糖尿病大鼠随机分为3组,分别为罗格列酮组、I/R损伤模型组、假手术组,灌胃给药1w后行I/R损伤手术,观察I/R损伤后心肌梗死面积,心律失常发生次数和类型以及血压的变化。结果罗格列酮组减少了心肌梗死面积与左心室面积比值(IS/LV,30.3±3.6vs38.7±3.0)以及心肌梗死面积与危险区面积比值(AAR/LV,76.0±2.6vs93.2±3.0),降低了心律失常评分(2.6±0.4vs4.4±0.8),改善了再灌注平均动脉压恢复,与I/R组比较均有显著性差异。结论罗格列酮对糖尿病大鼠心肌I/R损伤具有保护作用。

Objective To observe the effects of rosiglitazone on myocardial ischemia-reperfusion （I/R） in type 2 diabetic （T2DM） rats. Methods T2DM rat model was established by high lipid diet combined multiple low dose streptozocin （STZ）. I/R injury rat model was induced via ligating left anterior descending branch of coronary artery for 30 min and reperfusing for 120 min. T2DM rats were randomly divided into rosiglitazone, I/R and shame-operated groups. Myocardial infarct size （IS） and the ratio of IS and area at risk （AAR） were determined, arrhythmias score was evaluated according to Lambeth conventions, and blood pressure （BP） was recorded 1 w after intragastric administration and I/R injury operation. Results Compared with IR group, rosiglitazone decreased the ration of IS/LV （30. 3 ± 3. 6 vs 38.7 ± 3. 0） and the rate of AAR/LV （76. 0 ± 2. 6 vs 93.2 ± 3.0）, reduced scores of arrhythmias （2. 6 ± 0. 4 vs 4. 4 ± 0. 8 ） and ameliorated the restore of BP in reperfusion period （P 〈 0.05）. Conclusions Rosightazone has the protective effects on acute myocardial I/R injure in T2DM rats.