跑台运动训练促进脑梗死大鼠外源性移植的神经干细胞迁移分化的实验研究

Experimental study of the effects of treadmill training on promoting transplanted neural stem cell migration and differentiation in rat′s brain after cerebral infarction

目的:研究跑台运动训练促进移植到脑梗死大鼠纹状体内的神经干细胞存活、迁移和分化,以及神经功能障碍的康复。方法:选用大脑中动脉缺血大鼠模型,在缺血后第5天将培养的神经干细胞立体定向移植到缺血纹状体区。30只大鼠随机分为移植组、跑台运动训练组以及移植联合跑台运动训练组。移植组大鼠自由活动。分别在缺血前1天,缺血后第5,6,14和28天评测大鼠的神经行为学评分情况。免疫荧光染色观察神经干细胞在脑内的迁移、分化情况。结果:免疫荧光染色显示,运动训练的大鼠移植后的神经干细胞在宿主脑内存活、迁移分化情况较无运动训练而自由活动大鼠好。移植联合运动训练组大鼠的神经行为学评分较单纯移植和单纯运动组低(P<0.05,P<0.01)。结论:运动训练可以促进移植的神经干细胞迁移、分化,并提高移植细胞在脑梗死大鼠脑内的存活率,促进神经功能障碍的康复。

Objective：To study the effects of treadmill training on promoting transplanted neural stem cell survival,migration and differentiation in rat＇s brain after cerebral infarction, and improving the neurological dysfunction rehabilitation. Method： Middle cerebral artery ischemia model was used. Cultured neural stem cells were transplanted into ischemic striatum with stereotactic on the 5th day. Thirty rats were randomly divided into three groups： transplantation group, treadmill training group and transplantation combined with treadmill training group.On the day before ischemia, as well as the 5th, 6th, 14th and 28th day after ischemia all rats were evaluated with neurobehavioral assessment. Immunofluorescence staining was used to detect neural migration and differentiation of stem cells in the brain.Result： Immunofluorescence staining showed the rates of survival, migration and differentiation of transplanted neural stem cells in host＇s brain after cerebral infarction in treadmill training rates were better than those in free activities and no treadmill training rats The neurological behavioral scores in transplantation combined with treadmill training group were lower than those in transplantation alone and treadmill training alone groups repectively （P〈0.05, P〈0.01）. Conclusion： Treadmill training can promote migration, differentiation and survival of the transplanted neural stem cell in the brain after cerebral infarction and improve the rehabilitation outcome for neurological dysfunction.