肝癌高发区饮用塘水致大鼠肝癌作用长期实验研究

INDUCTION OF RAT HEPATOCARCINOMA BY POND WATER OF HIGH HUMAN HEPATOCARCINOMA INCIDENCE REGION

研究广西肝癌（ＨＣＣ）高发区居民饮用塘水致大鼠ＨＣＣ作用。实验采用黄曲霉毒素Ｂ１（ＡＦＢ１）致大鼠肝癌模型，Ｗｉｓｔａｒ大鼠１６０只，随机分为４组：Ａ组为空白对照组；Ｂ组注射ＡＦＢ１；Ｃ组饲０．２％塘水浓缩物；Ｄ组注射ＡＦＢ１和饲０．２％塘水浓缩物，检测γ－谷氨酰转肽酶阳性肝细胞增灶（ＧＧＴ阳性灶）和ＨＣＣ发生情况。ＧＧＴ阳性灶用电脑图像分析系统统计平面及立体指标。结果：Ｄ组ＧＧＴ阳性灶发生最多，ＨＣＣ发生率最高（４２．１％）；Ｃ组ＧＧＴ阳性灶比空白组多且大（Ｐ＜０．０５），但远少于Ｂ组（Ｐ＜０．０１）；Ｂ组ＨＣＣ发生率为２３．８％，Ａ、Ｃ两组无ＨＣＣ发生。提示：广西肝癌高发区饮用塘水中存在与ＨＣＣ发生相关的致癌或促癌物质，其作用弱于ＡＦＢ１，暴露于ＡＦＢ１同时饮用塘水可能增加罹患ＨＣＣ危险性。

The effects of drinking pond water of Guangxi high human hepatocarcinoma incidence area in the induction of hepatocarcinoma were studied by using AFB1induced rats HCC model160 Wistar rats were divided into 4 groups:Group A acted as controls;Group B received AFB1 injections;Group C ate 02% pond water concentrates obtained from a high HCC incidence area in Guangxi;Group D received both AFB1 injections and 02% pond water concentratesResults:①Group D had the greatest number of γglutamyltranspeptidase(GGT+) foci,and the highest hepatocarcinoma incidence (421%)Although the number of GGT+foci of Group C was lesser than that of Group B (P<001),but was higher than that of control Group A (P<005)This investigation suggests that some kind of hepatocarcinogens may exist in such pond waterThus,exposure to AFB1 in combination with drinking contaminated pond water may increase the danger of inducing HCC