中药解毒生肌膏治疗大鼠糖尿病足的实验研究

An experimental study on treatment of diabetic foot ulcer in rats by antidotal and gynogenic ointment of traditional Chinese medicine (中药解毒生肌膏)

目的研究中药解毒生肌膏治疗大鼠糖尿病足疗效,并探讨其临床应用价值。方法腹腔注射链脲佐菌素55 mg/kg制备Wistar大鼠糖尿病模型。喂养6个月时结扎大鼠腘动脉下端,7个月时对糖尿病大鼠后肢足背皮肤进行烫伤,Wagner分级为Ⅲ级。按随机数字表法将40只大鼠均分为糖尿病足溃疡常规治疗组(对照组)和中药解毒生肌膏治疗组(实验组)。实验组局部应用中药解毒生肌膏剂外敷,对照组应用重组人表皮生长因子外用液,每2 d换药1次,连续30 d。观察两组治疗前后Wagner分级、溃疡大小、创面肉芽生长情况、愈合时间及药物毒副作用。结果实验组总有效率(70.0%,14/20)、治愈率(50.0%,10/20)均显著高于对照组(40.0%,8/20;15.0%,3/20),差异均有统计学意义(P均<0.05)。两组治疗后溃疡大小和创面肉芽组织陈旧率均较治疗前有不同程度改善,以实验组效果更为显著(P均<0.05);且实验组溃疡愈合时间明显短于对照组[(10.2±3.5)d比(16.2±3.5)d,P<0.05]。两组治疗前后均未出现药物毒副作用。结论中药解毒生肌膏可以改善大鼠糖尿病足的微循环,缩短足溃疡愈合时间。

Objective To explore the efficacy of antidotal and gynogenic ointment of traditional Chinese medicine （CMAGO,中药解毒生肌膏 ） on rat diabetic foot and its value of clinical application in future. Methods Wistar rats were injected intraperitoneally with streptozocin （STZ, 55 mg/kg body weight） once to induce models of diabetes. After the rat was raised for 6 months, the popliteal artery was ligated at the distal end, and after 7 months of raising, the dorsal foot skin of the hind extremity of the diabetic rat was scalded. The degree of scald reached to grade ; in the Wagner classification. Forty rats were randomized equally by random number table into diabetic foot ulcer conventional treatment group and CMAGO experimental group. The rats in experimental group were treated by the local external application of CMAGO and the rats in conventional treatment group were treated by the local external application of a solution, Recombinant Human Epidermal Growth Factor. The dressing was changed once every two days for 30 days. Then the Wagner grade, ulcer size, growth of granulation, time of healing in the two groups were observed before and after treatment, and the side effects of the drugs were noted. Results The total efficiency （70. 0%, 14/20） and the cure rate （50. 0%, 10/20） of the experimental group were significantly higher than those of the conventional group （40.0%, 8/20; 15.0% 3/20）, the differences being statistically significant （both P〈0.05）. Compared with those before the treatment, after treatment in the two groups, the size of the ulcer and the situation of the granulation tissue were improved in various degrees, the therapeutic effects being markedly better in the experimental group （all P〈0. 05）. The healing time in the experimental group was significantly shorter than that in the conventional group [（10. 2±3. 5） days vs. （16.2±3.5） days, P〈0. 05]. The side effects of the drugs in the two groups were not seen. Conclusion CMAGO can improve the micro-circulation of the rat diabetic foot and shorten the healing time of the foot ulcer.