大鼠实验性血栓模型建立的新方法

The new method of form the thrombus former get from the mice experiment

目的为研究血栓性疾病而探索一种简便易行的创建血栓模型新的机械性损伤血管内膜方法及可行性。方法新西兰大鼠60只,均分为A、B、C3组,分别以银针钳夹法、注胰蛋白酶法、传统损伤血管内膜法制备动物血栓模型,3h为实验终点。结果新的损伤血管内膜法制备的动脉血栓成功率达86%,胰蛋白酶法成功率80%,传统损伤内膜法成功率达58%,其成功率A组高于B、C组,并且A组与C组比较,呈明显差异(P<0.01),A组与B组比较无显著性差异(P<0.05)。银针钳夹法,方法简便、易行,节省实验时间。结论银针钳夹法即银针插入动脉内,用止血钳从不同角度、均匀外力钳夹动脉血管方法建立动脉血栓模型,通过本实验评价具有科学性、实用性、可行性。

Objective for research thrombus diseases and explore a sort of simple method with feasibilities about form the thrombus former of a new mechanical harm the velum inside blood vessels. Methods 60 New Zealand mice, allot to A, B, C three groups, use silver acus clamp method.trypsin method.tradition method of harm the velum inside blood vessels to prepare the animal thrombus former,3 h is the end of the experiment. Results The new method of harm the velum inside blood vessels to prepare the animal thrombus former, it＇s success efficiency reach to 86 %, trypsin method reach to 80%, tradition method of harm the velum inside blood vessels reach to 58% ,A group＇s success efficiency better than B.C group, and to compare A group and C group,it shows obvious difference（ P 〈 0.01）, to compare Agroup and B group there has no prominent difference（ P 〉 0.05）. silver acus clamp method are simple, convenient, save experiment time. Conclusion silver acus clamp method are steel wire or silver acus to insert the artery part, use hemostat from different angle,equable and outside force to vice artery vas and form the thrombus former, through the appraise of this experiment it has＇ provided with scientitlc.practicability.feasibility.