尿草酸对肾草钙结石形成和肾组织自由基影响的研究

The effect of oxalate in urine on calcium oxalate crystallizations and free radicals in rat kidney

目的了解尿草酸浓度对大鼠肾草酸钙结石形成和肾组织自由基相关指标的影响。方法将1%、0.75%和0.5%乙二醇溶液和去离子水分别作为四组SD大鼠的唯一饮用水源,观察并比较第0、2、4、6周末时,各组大鼠的尿草酸浓度,24h尿草酸排泄量、肾组织草酸钙结晶沉积情况、肾组织脂质过氧化物丙二醛(MDA)及超氧化物歧化酶(SOD)水平。结果随着大鼠尿草酸浓度的升高,肾组织MDA水平明显升高,SOD水平明显下降,最终导致肾组织草酸钙结晶沉积明增多。结论尿草酸能使肾组织产生过量自由基,而自由基的增加与肾草酸钙结石的形成有密切的关系。

Objective To investigate the effect of oxalate urine on calcium oxalate crystallizations and free radicals in rat kidneynethods The male Sprague-Dawiey rats were randomly divided into 4 groups, ie, 0.5 % ,0.75 %, 1% ethylene glycol group and control group. 0, 2, 4,6 weeklater, the rats were sacrificed and the urine oxalate concentration, the 24 hours urine oxalate excretion, the calcium oxalate crystallization in kidney,the renal free radical level were detectedResults With the urine oxalate concentration increasing, MDA was increased and SOD was reduced. At the end the calcium oxalate crystallization in rat kidney was increased obviously Conclusion The urine oxalate can induce the renal free radical creation excessively. The calcium oxalate crystallization formation in rat kidney has a significant relationship with the renal free radical level increasing