摘要
目的将全长外源多药耐药基因转入间充质干细胞使其外源基因得以表达。方法从胎盘组织中分离培养间充质干细胞,构建携带全长外源多药耐药基因的逆转录病毒,将此逆转录病毒转染间充质干细胞,绿色荧光显微镜检测外源基因的表达,western blot检测MDR1基因表达产物。结果绿色荧光显微镜可观测到转染后间充质干细胞有绿色荧光蛋白的表达,western blot检测到全长多药耐药基因编码的p-糖蛋白的表达。结论mdr1逆转录病毒载体可有效转染人胎盘源MSC,转染的外源基因可充分表达。
Objective To investigate the expression of MDR1 gene in the mesenchymal stem cells transfected. Methods a retrovirus vector taking mdrlgene as exogenous gene and GFP gene as report gene was constructed, ex vivo, they were deliveried into MSCs derived from human placenta.Results green fluorescent proteins were observed by fluorescence microscope after transfection, so it was tested indirectly that mdrl gene had been expressed. In Westeru Blot Test, MSCs pMX-ABCB1 was p-gp possitive at the marker 140 Kda. Conclusion MSCs derived from human placenta can be transfected by PMX-MDR1 successfully, and exogentic mdrl gene can be expressed.
出处
《中国实验诊断学》
2008年第7期857-859,共3页
Chinese Journal of Laboratory Diagnosis
基金
国家自然科学基金资助课题(30471804)
